Show simple item record

dc.contributor.authorKind, Jop
dc.contributor.authorPagie, Ludo
dc.contributor.authorde Vries, Sandra S.
dc.contributor.authorNahidiazar, Leila
dc.contributor.authorDey, Siddharth S.
dc.contributor.authorBienko, Magda
dc.contributor.authorZhan, Ye
dc.contributor.authorLajoie, Bryan
dc.contributor.authorde Graaf, Carolyn A.
dc.contributor.authorAmendola, Mario
dc.contributor.authorFudenberg, Geoffrey
dc.contributor.authorImakaev, Maxim
dc.contributor.authorMirny, Leonid A.
dc.contributor.authorJalink, Kees
dc.contributor.authorDekker, Job
dc.contributor.authorvan Oudenaarden, Alexander
dc.contributor.authorvan Steensel, Bas
dc.date2022-08-11T08:11:00.000
dc.date.accessioned2022-08-23T17:27:51Z
dc.date.available2022-08-23T17:27:51Z
dc.date.issued2015-09-24
dc.date.submitted2015-10-29
dc.identifier.citationCell. 2015 Sep 24;163(1):134-47. doi: 10.1016/j.cell.2015.08.040. Epub 2015 Sep 10. <a href="http://dx.doi.org/10.1016/j.cell.2015.08.040">Link to article on publisher's site</a>
dc.identifier.issn0092-8674 (Linking)
dc.identifier.doi10.1016/j.cell.2015.08.040
dc.identifier.pmid26365489
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49957
dc.description.abstractMammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26365489&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.cell.2015.08.040
dc.subjectGenomics
dc.subjectMolecular Biology
dc.subjectSystems Biology
dc.titleGenome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
dc.typeJournal Article
dc.source.journaltitleCell
dc.source.volume163
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/sysbio_pubs/77
dc.identifier.contextkey7778185
html.description.abstract<p>Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.</p>
dc.identifier.submissionpathsysbio_pubs/77
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentProgram in Systems Biology
dc.source.pages134-47


Files in this item

Thumbnail
Name:
Publisher version

This item appears in the following Collection(s)

Show simple item record