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dc.contributor.authorFeng, Huiyun
dc.contributor.authorReece-Hoyes, John S.
dc.contributor.authorWalhout, Albertha J. M.
dc.contributor.authorHope, Ian A.
dc.date2022-08-11T08:11:00.000
dc.date.accessioned2022-08-23T17:27:54Z
dc.date.available2022-08-23T17:27:54Z
dc.date.issued2012-02-15
dc.date.submitted2012-08-10
dc.identifier.citationGene. 2012 Feb 15;494(1):73-84. Epub 2011 Dec 19. <a href="http://dx.doi.org/10.1016/j.gene.2011.11.042">Link to article on publisher's site</a>
dc.identifier.issn0378-1119 (Linking)
dc.identifier.doi10.1016/j.gene.2011.11.042
dc.identifier.pmid22207033
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49967
dc.description.abstractHomeobox proteins are critical regulators of developmental gene transcription and cell specification. Many insights into transcriptional regulation have been gained from studies in the nematode Caenorhabditis elegans. We investigated the expression and regulation of the C. elegans homeobox gene ceh-63, which encodes a single-homeodomain transcription factor of 152 amino acids. ceh-63 is expressed in the interneuron DVC in both sexes, from late embryogenesis through adulthood, and two pairs of uterine cells in reproductive hermaphrodites only. A reporter gene fusion, encoding GFP fused to the full-length CEH-63, also drove weak inconsistent expression in additional unidentified cells in the head and tail. A potential ceh-63 null mutant had no obvious abnormalities, except for a possible increase in subtle defects of the DVC axon projection. No behavioural responses were observed upon either laser ablation of DVC or activation of DVC through light stimulation of channelrhodopsin-2 specifically expressed in this neuron. The function of DVC therefore remains enigmatic. A transcriptional regulatory cascade operating in DVC was defined from the LIM-homeodomain protein CEH-14 through CEH-63 to the helix-turn-helix transcription factor MBR-1. Both CEH-14 and CEH-63 individually bound the mbr-1 promoter in a yeast one-hybrid assay. A model is proposed suggesting that CEH-14 activates ceh-63 and then along with CEH-63 co-ordinately activates mbr-1.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22207033&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.gene.2011.11.042
dc.subjectAnimals
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subjectFemale
dc.subjectGene Expression Regulation, Developmental
dc.subjectHomeodomain Proteins
dc.subjectInterneurons
dc.subjectLIM-Homeodomain Proteins
dc.subjectMale
dc.subjectTranscription Factors
dc.subjectGenetics and Genomics
dc.subjectSystems Biology
dc.titleA regulatory cascade of three transcription factors in a single specific neuron, DVC, in Caenorhabditis elegans
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume494
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/sysbio_pubs/9
dc.identifier.contextkey3201826
html.description.abstract<p>Homeobox proteins are critical regulators of developmental gene transcription and cell specification. Many insights into transcriptional regulation have been gained from studies in the nematode Caenorhabditis elegans. We investigated the expression and regulation of the C. elegans homeobox gene ceh-63, which encodes a single-homeodomain transcription factor of 152 amino acids. ceh-63 is expressed in the interneuron DVC in both sexes, from late embryogenesis through adulthood, and two pairs of uterine cells in reproductive hermaphrodites only. A reporter gene fusion, encoding GFP fused to the full-length CEH-63, also drove weak inconsistent expression in additional unidentified cells in the head and tail. A potential ceh-63 null mutant had no obvious abnormalities, except for a possible increase in subtle defects of the DVC axon projection. No behavioural responses were observed upon either laser ablation of DVC or activation of DVC through light stimulation of channelrhodopsin-2 specifically expressed in this neuron. The function of DVC therefore remains enigmatic. A transcriptional regulatory cascade operating in DVC was defined from the LIM-homeodomain protein CEH-14 through CEH-63 to the helix-turn-helix transcription factor MBR-1. Both CEH-14 and CEH-63 individually bound the mbr-1 promoter in a yeast one-hybrid assay. A model is proposed suggesting that CEH-14 activates ceh-63 and then along with CEH-63 co-ordinately activates mbr-1.</p>
dc.identifier.submissionpathsysbio_pubs/9
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages73-84


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