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dc.contributor.authorKnuckley, Bryan
dc.contributor.authorJones, Justin E.
dc.contributor.authorBachovchin, Daniel A.
dc.contributor.authorSlack, Jessica
dc.contributor.authorCausey, Corey P.
dc.contributor.authorBrown, Steven J.
dc.contributor.authorRosen, Hugh
dc.contributor.authorCravatt, Benjamin F.
dc.contributor.authorThompson, Paul R
dc.date2022-08-11T08:11:00.000
dc.date.accessioned2022-08-23T17:28:18Z
dc.date.available2022-08-23T17:28:18Z
dc.date.issued2010-10-14
dc.date.submitted2015-05-27
dc.identifier.citationChem Commun (Camb). 2010 Oct 14;46(38):7175-7. doi: 10.1039/c0cc02634d. <a href="http://dx.doi.org/10.1039/c0cc02634d">Link to article on publisher's site</a>. Epub 2010 Aug 25.
dc.identifier.issn1359-7345 (Linking)
dc.identifier.doi10.1039/c0cc02634d
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50049
dc.description<p>At the time of publication, Paul Thompson was not yet affiliated with UMass Medical School.</p>
dc.description.abstractProtein Arginine Deiminase (PAD) activity is dysregulated in numerous diseases, e.g., Rheumatoid Arthritis. Herein we describe the development of a fluorescence polarization-Activity Based Protein Profiling (fluopol-ABPP) based high throughput screening assay that can be used to identify PAD-selective inhibitors. Using this assay, streptonigrin was identified as a potent, selective, and irreversible PAD4 inactivator.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20740228&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943038/
dc.subjectArthritis, Rheumatoid
dc.subjectCell Line, Tumor
dc.subjectDrug Evaluation, Preclinical
dc.subjectEnzyme Inhibitors
dc.subjectFluorescence Polarization
dc.subjectFluorescent Dyes
dc.subjectHigh-Throughput Screening Assays
dc.subjectHumans
dc.subjectHydrolases
dc.subjectInhibitory Concentration 50
dc.subjectStreptonigrin
dc.subjectBiochemistry
dc.subjectEnzymes and Coenzymes
dc.subjectMedicinal-Pharmaceutical Chemistry
dc.subjectTherapeutics
dc.titleA fluopol-ABPP HTS assay to identify PAD inhibitors
dc.typeJournal Article
dc.source.journaltitleChemical communications (Cambridge, England)
dc.source.volume46
dc.source.issue38
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1056&amp;context=thompson&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/thompson/57
dc.identifier.contextkey7144791
refterms.dateFOA2022-08-23T17:28:18Z
html.description.abstract<p>Protein Arginine Deiminase (PAD) activity is dysregulated in numerous diseases, e.g., Rheumatoid Arthritis. Herein we describe the development of a fluorescence polarization-Activity Based Protein Profiling (fluopol-ABPP) based high throughput screening assay that can be used to identify PAD-selective inhibitors. Using this assay, streptonigrin was identified as a potent, selective, and irreversible PAD4 inactivator.</p>
dc.identifier.submissionpaththompson/57
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages7175-7


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