Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice
Schiller, Zachary A.
Wise, Megan C.
Broderick, Kate E.
Weiner, David B.
Klempner, Mark S.
UMass Chan AffiliationsMassBiologics
Document TypeJournal Article
human monoclonal antibody
Bacterial Infections and Mycoses
Immunology of Infectious Disease
Immunoprophylaxis and Therapy
Translational Medical Research
MetadataShow full item record
AbstractWe recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of > 6 mug/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.
Wang Y, Esquivel R, Flingai S, Schiller ZA, Kern A, Agarwal S, Chu J, Patel A, Sullivan K, Wise MC, Broderick KE, Hu L, Weiner DB, Klempner MS. Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice. J Infect Dis. 2019 Mar 15;219(7):1146-1150. doi: 10.1093/infdis/jiy627. PMID: 30476132; PMCID: PMC6420172. Link to article on publisher's site