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dc.contributor.authorWang, Yang
dc.contributor.authorEsquivel, Rianne
dc.contributor.authorFlingai, Seleeke
dc.contributor.authorSchiller, Zachary A.
dc.contributor.authorKern, Aurelie
dc.contributor.authorAgarwal, Sangya
dc.contributor.authorChu, Jacqueline
dc.contributor.authorPatel, Ami
dc.contributor.authorSullivan, Katherine
dc.contributor.authorWise, Megan C.
dc.contributor.authorBroderick, Kate E.
dc.contributor.authorHu, Linden
dc.contributor.authorWeiner, David B.
dc.contributor.authorKlempner, Mark S.
dc.date2022-08-11T08:11:02.000
dc.date.accessioned2022-08-23T17:29:43Z
dc.date.available2022-08-23T17:29:43Z
dc.date.issued2019-03-15
dc.date.submitted2020-02-20
dc.identifier.citation<p>Wang Y, Esquivel R, Flingai S, Schiller ZA, Kern A, Agarwal S, Chu J, Patel A, Sullivan K, Wise MC, Broderick KE, Hu L, Weiner DB, Klempner MS. Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice. J Infect Dis. 2019 Mar 15;219(7):1146-1150. doi: 10.1093/infdis/jiy627. PMID: 30476132; PMCID: PMC6420172. <a href="https://doi.org/10.1093/infdis/jiy627">Link to article on publisher's site</a></p>
dc.identifier.issn0022-1899 (Linking)
dc.identifier.doi10.1093/infdis/jiy627
dc.identifier.pmid30476132
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50384
dc.description.abstractWe recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of > 6 mug/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30476132&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420172/
dc.subjectDMAb
dc.subjectLyme disease
dc.subjectOspA
dc.subjecthuman monoclonal antibody
dc.subjectUMCCTS funding
dc.subjectBacteria
dc.subjectBacterial Infections and Mycoses
dc.subjectImmunity
dc.subjectImmunology of Infectious Disease
dc.subjectImmunoprophylaxis and Therapy
dc.subjectTranslational Medical Research
dc.titleAnti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice
dc.typeJournal Article
dc.source.journaltitleThe Journal of infectious diseases
dc.source.volume219
dc.source.issue7
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/umccts_pubs/210
dc.identifier.contextkey16603956
html.description.abstract<p>We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of > 6 mug/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.</p>
dc.identifier.submissionpathumccts_pubs/210
dc.contributor.departmentMassBiologics
dc.source.pages1146-1150


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