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    Mouse Dux is myotoxic and shares partial functional homology with its human paralog DUX4

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    Authors
    Eidahl, Jocelyn O.
    Giesige, Carlee R.
    Domire, Jacqueline S.
    Wallace, Lindsay M.
    Fowler, Allison M.
    Guckes, Susan M.
    Garwick-Coppens, Sara E.
    Labhart, Paul
    Harper, Scott Q.
    UMass Chan Affiliations
    Wellstone Center for FSHD
    Document Type
    Journal Article
    Publication Date
    2016-10-15
    Keywords
    Cell Biology
    Developmental Biology
    Molecular Biology
    Molecular Genetics
    Musculoskeletal Diseases
    Nervous System Diseases
    
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    Link to Full Text
    https://doi.org/10.1093/hmg/ddw287
    Abstract
    D4Z4 repeats are present in at least 11 different mammalian species, including humans and mice. Each repeat contains an open reading frame encoding a double homeodomain (DUX) family transcription factor. Aberrant expression of the D4Z4 ORF called DUX4 is associated with the pathogenesis of Facioscapulohumeral muscular dystrophy (FSHD). DUX4 is toxic to numerous cell types of different species, and over-expression caused dysmorphism and developmental arrest in frogs and zebrafish, embryonic lethality in transgenic mice, and lesions in mouse muscle. Because DUX4 is a primate-specific gene, questions have been raised about the biological relevance of over-expressing it in non-primate models, as DUX4 toxicity could be related to non-specific cellular stress induced by over-expressing a DUX family transcription factor in organisms that did not co-evolve its regulated transcriptional networks. We assessed toxic phenotypes of DUX family genes, including DUX4, DUX1, DUX5, DUXA, DUX4-s, Dux-bl and mouse Dux. We found that DUX proteins were not universally toxic, and only the mouse Dux gene caused similar toxic phenotypes as human DUX4. Using RNA-seq, we found that 80% of genes upregulated by Dux were similarly increased in DUX4-expressing cells. Moreover, 43% of Dux-responsive genes contained ChIP-seq binding sites for both Dux and DUX4, and both proteins had similar consensus binding site sequences. These results suggested DUX4 and Dux may regulate some common pathways, and despite diverging from a common progenitor under different selective pressures for millions of years, the two genes maintain partial functional homology.
    Source
    Hum Mol Genet. 2016 Oct 15;25(20):4577-4589. doi: 10.1093/hmg/ddw287. Link to article on publisher's site
    DOI
    10.1093/hmg/ddw287
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/50572
    PubMed ID
    28173143
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1093/hmg/ddw287
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    UMass Chan Faculty and Researcher Publications
    Wellstone Center for FSHD Publications

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