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dc.contributor.authorMao, C. S.
dc.contributor.authorStavnezer, Janet
dc.date2022-08-11T08:11:04.000
dc.date.accessioned2022-08-23T17:31:07Z
dc.date.available2022-08-23T17:31:07Z
dc.date.issued2001-08-01
dc.date.submitted2007-09-14
dc.identifier.citation<p>J Immunol. 2001 Aug 1;167(3):1522-34.</p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.doi10.4049/jimmunol.167.3.1522
dc.identifier.pmid11466373
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50660
dc.description.abstractBefore Ig class switching, RNA transcription through the specific S regions undergoing recombination is induced by cytokines and other activators that induce and direct switching. The resulting germline (GL) transcripts are essential for switch recombination. To understand the differential regulation of mouse IgG1 and IgE, we compared the promoters for GL gamma1 and epsilon transcripts. We addressed the question of why the promoter that regulates GL epsilon transcription is more responsive to IL-4 than the gamma1 promoter and also why GL epsilon transcription is more dependent on IL-4 than is gamma1 transcription. We found that the IL-4-responsive region of the GL epsilon promoter is more inducible than that of the gamma1 promoter, although each promoter contains a binding site for the IL-4-inducible transcription factor Stat6, located immediately adjacent to a binding site for a basic region leucine zipper (bZip) family protein. However, the arrangement and sequences of the sites differ between the epsilon and gamma1 promoters. The GL epsilon promoter binds Stat6 with a 10-fold higher affinity than does the gamma1 promoter. Furthermore, the bZip elements of the two promoters bind different transcription factors, as the GL epsilon promoter binds and is activated by AP-1, whereas the gamma1 promoter binds and is activated by activating transcription factor 2. C/EBPbeta and C/EBPgamma also bind the gamma1 bZip element, although they inhibit rather than activate transcription. However, inhibition of promoter activity by C/EBPbeta does not require the bZip element and may instead occur via inhibiting the activity of NF-kappaB.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11466373&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.4049/jimmunol.167.3.1522
dc.subjectActivating Transcription Factor 2
dc.subjectActivating Transcription Factors
dc.subjectAnimals
dc.subjectAntigens, CD40
dc.subjectB-Lymphocytes
dc.subjectBase Sequence
dc.subjectBasic-Leucine Zipper Transcription Factors
dc.subjectBinding Sites
dc.subjectBlood Proteins
dc.subjectCCAAT-Enhancer-Binding Proteins
dc.subjectCell Line
dc.subjectCell Nucleus
dc.subjectCyclic AMP Response Element-Binding
dc.subjectProtein
dc.subjectDNA-Binding Proteins
dc.subjectG-Box Binding Factors
dc.subjectGene Expression Regulation
dc.subjectImmunoglobulin epsilon-Chains
dc.subjectImmunoglobulin gamma-Chains
dc.subjectInterleukin-4
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred C57BL
dc.subjectMolecular Sequence Data
dc.subjectNF-kappa B
dc.subjectPromoter Regions (Genetics)
dc.subjectResponse Elements
dc.subjectSTAT6 Transcription Factor
dc.subjectSignal Transduction
dc.subjectSpleen
dc.subjectTrans-Activation (Genetics)
dc.subjectTrans-Activators
dc.subjectTranscription Factors
dc.subjectTumor Cells, Cultured
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectWomen's Studies
dc.titleDifferential regulation of mouse germline Ig gamma 1 and epsilon promoters by IL-4 and CD40
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume167
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/wfc_pp/186
dc.identifier.contextkey367647
html.description.abstract<p>Before Ig class switching, RNA transcription through the specific S regions undergoing recombination is induced by cytokines and other activators that induce and direct switching. The resulting germline (GL) transcripts are essential for switch recombination. To understand the differential regulation of mouse IgG1 and IgE, we compared the promoters for GL gamma1 and epsilon transcripts. We addressed the question of why the promoter that regulates GL epsilon transcription is more responsive to IL-4 than the gamma1 promoter and also why GL epsilon transcription is more dependent on IL-4 than is gamma1 transcription. We found that the IL-4-responsive region of the GL epsilon promoter is more inducible than that of the gamma1 promoter, although each promoter contains a binding site for the IL-4-inducible transcription factor Stat6, located immediately adjacent to a binding site for a basic region leucine zipper (bZip) family protein. However, the arrangement and sequences of the sites differ between the epsilon and gamma1 promoters. The GL epsilon promoter binds Stat6 with a 10-fold higher affinity than does the gamma1 promoter. Furthermore, the bZip elements of the two promoters bind different transcription factors, as the GL epsilon promoter binds and is activated by AP-1, whereas the gamma1 promoter binds and is activated by activating transcription factor 2. C/EBPbeta and C/EBPgamma also bind the gamma1 bZip element, although they inhibit rather than activate transcription. However, inhibition of promoter activity by C/EBPbeta does not require the bZip element and may instead occur via inhibiting the activity of NF-kappaB.</p>
dc.identifier.submissionpathwfc_pp/186
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages1522-34


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