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    Activation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors

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    Authors
    Shen, Ching-Hung
    Stavnezer, Janet
    UMass Chan Affiliations
    Department of Molecular Genetics and Microbiology
    Document Type
    Journal Article
    Publication Date
    2001-01-01
    Keywords
    Adjuvants, Immunologic
    Animals
    B-Lymphocytes
    Base Sequence
    CCAAT-Enhancer-Binding Proteins
    CD40 Ligand
    Cell Line
    Cells, Cultured
    DNA-Binding Proteins
    Gene Expression Regulation
    Genes, Reporter
    Half-Life
    Humans
    Immunoglobulin epsilon-Chains
    Interleukin-4
    Kinetics
    Lymphocyte Activation
    Mice
    Mice, Inbred BALB C
    Molecular Sequence Data
    Multigene Family
    Plasmids
    Promoter Regions (Genetics)
    Proto-Oncogene Proteins c-fos
    Proto-Oncogene Proteins c-jun
    STAT6 Transcription Factor
    Signal Transduction
    Spleen
    Trans-Activators
    Transcription Factor AP-1
    inhibitors
    Transfection
    Tumor Cells, Cultured
    Life Sciences
    Medicine and Health Sciences
    Women's Studies
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    Link to Full Text
    https://doi.org/10.4049/jimmunol.166.1.411
    Abstract
    Induction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6.
    Source

    J Immunol. 2001 Jan 1;166(1):411-23. Link to article on publisher's site

    DOI
    10.4049/jimmunol.166.1.411
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/50663
    PubMed ID
    11123319
    Related Resources

    Link to article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.4049/jimmunol.166.1.411
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