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dc.contributor.authorShen, Ching-Hung
dc.contributor.authorStavnezer, Janet
dc.date2022-08-11T08:11:04.000
dc.date.accessioned2022-08-23T17:31:08Z
dc.date.available2022-08-23T17:31:08Z
dc.date.issued2001-01-01
dc.date.submitted2007-09-14
dc.identifier.citation<p>J Immunol. 2001 Jan 1;166(1):411-23. <a href="http://www.jimmunol.org/cgi/reprint/166/1/411">Link to article on publisher's site</a></p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.doi10.4049/jimmunol.166.1.411
dc.identifier.pmid11123319
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50663
dc.description.abstractInduction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11123319&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.4049/jimmunol.166.1.411
dc.subjectAdjuvants, Immunologic
dc.subjectAnimals
dc.subjectB-Lymphocytes
dc.subjectBase Sequence
dc.subjectCCAAT-Enhancer-Binding Proteins
dc.subjectCD40 Ligand
dc.subjectCell Line
dc.subjectCells, Cultured
dc.subjectDNA-Binding Proteins
dc.subjectGene Expression Regulation
dc.subjectGenes, Reporter
dc.subjectHalf-Life
dc.subjectHumans
dc.subjectImmunoglobulin epsilon-Chains
dc.subjectInterleukin-4
dc.subjectKinetics
dc.subjectLymphocyte Activation
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectPlasmids
dc.subjectPromoter Regions (Genetics)
dc.subjectProto-Oncogene Proteins c-fos
dc.subjectProto-Oncogene Proteins c-jun
dc.subjectSTAT6 Transcription Factor
dc.subjectSignal Transduction
dc.subjectSpleen
dc.subjectTrans-Activators
dc.subjectTranscription Factor AP-1
dc.subjectinhibitors
dc.subjectTransfection
dc.subjectTumor Cells, Cultured
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectWomen's Studies
dc.titleActivation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume166
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/wfc_pp/189
dc.identifier.contextkey367650
html.description.abstract<p>Induction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6.</p>
dc.identifier.submissionpathwfc_pp/189
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages411-23


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