Activation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors
dc.contributor.author | Shen, Ching-Hung | |
dc.contributor.author | Stavnezer, Janet | |
dc.date | 2022-08-11T08:11:04.000 | |
dc.date.accessioned | 2022-08-23T17:31:08Z | |
dc.date.available | 2022-08-23T17:31:08Z | |
dc.date.issued | 2001-01-01 | |
dc.date.submitted | 2007-09-14 | |
dc.identifier.citation | <p>J Immunol. 2001 Jan 1;166(1):411-23. <a href="http://www.jimmunol.org/cgi/reprint/166/1/411">Link to article on publisher's site</a></p> | |
dc.identifier.issn | 0022-1767 (Print) | |
dc.identifier.doi | 10.4049/jimmunol.166.1.411 | |
dc.identifier.pmid | 11123319 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/50663 | |
dc.description.abstract | Induction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11123319&dopt=Abstract">Link to article in PubMed</a></p> | |
dc.relation.url | https://doi.org/10.4049/jimmunol.166.1.411 | |
dc.subject | Adjuvants, Immunologic | |
dc.subject | Animals | |
dc.subject | B-Lymphocytes | |
dc.subject | Base Sequence | |
dc.subject | CCAAT-Enhancer-Binding Proteins | |
dc.subject | CD40 Ligand | |
dc.subject | Cell Line | |
dc.subject | Cells, Cultured | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Gene Expression Regulation | |
dc.subject | Genes, Reporter | |
dc.subject | Half-Life | |
dc.subject | Humans | |
dc.subject | Immunoglobulin epsilon-Chains | |
dc.subject | Interleukin-4 | |
dc.subject | Kinetics | |
dc.subject | Lymphocyte Activation | |
dc.subject | Mice | |
dc.subject | Mice, Inbred BALB C | |
dc.subject | Molecular Sequence Data | |
dc.subject | Multigene Family | |
dc.subject | Plasmids | |
dc.subject | Promoter Regions (Genetics) | |
dc.subject | Proto-Oncogene Proteins c-fos | |
dc.subject | Proto-Oncogene Proteins c-jun | |
dc.subject | STAT6 Transcription Factor | |
dc.subject | Signal Transduction | |
dc.subject | Spleen | |
dc.subject | Trans-Activators | |
dc.subject | Transcription Factor AP-1 | |
dc.subject | inhibitors | |
dc.subject | Transfection | |
dc.subject | Tumor Cells, Cultured | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.subject | Women's Studies | |
dc.title | Activation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of immunology (Baltimore, Md. : 1950) | |
dc.source.volume | 166 | |
dc.source.issue | 1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/wfc_pp/189 | |
dc.identifier.contextkey | 367650 | |
html.description.abstract | <p>Induction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6.</p> | |
dc.identifier.submissionpath | wfc_pp/189 | |
dc.contributor.department | Department of Molecular Genetics and Microbiology | |
dc.source.pages | 411-23 |