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dc.contributor.authorStavnezer, Janet
dc.contributor.authorMarcu, K. B.
dc.contributor.authorSirlin, S.
dc.contributor.authorAlhadeff, B.
dc.contributor.authorHammerling, U.
dc.date2022-08-11T08:11:04.000
dc.date.accessioned2022-08-23T17:31:22Z
dc.date.available2022-08-23T17:31:22Z
dc.date.issued1982-08-01
dc.date.submitted2007-09-14
dc.identifier.citation<p>Mol Cell Biol. 1982 Aug;2(8):1002-13.</p>
dc.identifier.issn0270-7306 (Print)
dc.identifier.doi10.1128/MCB.2.8.1002
dc.identifier.pmid6290869
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50716
dc.description.abstractThe B cell lymphoma I.29 consists of a mixture of cells expressing membrane-bound immunoglobulin M (IgM) (lambda) and IgA (lambda) of identical idiotypes. Whereas most of the cells express either IgM or IgA alone, 1 to 5% of the cells in this tumor express IgM and IgA simultaneously within the cytoplasm and on the cell membrane (R. Sitia et al., J. Immunol. 127:1388-1394, 1981; R. Sitia, unpublished data). When IgM+ cells are purified from the lymphoma and passaged in mice or cultured, a portion of the cells convert to IgA+. These properties suggest that some cells of the I.29 lymphoma may undergo immunoglobulin heavy chain switching, although it is also possible that the mixed population was derived by a prior switching event in a clone of cells. We performed Southern blotting experiments on genomic DNAs isolated from populations of I.29 cells containing variable proportions of IgM+ and IgA+ cells and on a number of cell lines derived from the lymphoma. The results were consistent with the deletion model for heavy chain switching, as the IgM+ cells contained rearranged mu genes and alpha genes in the germ line configuration on both the expressed and nonexpressed heavy chain chromosomes, whereas the IgA+ cells had deleted both mu genes and contained one rearranged and one germ line alpha gene. In addition, segments of DNA located within the intervening sequence 5' to the mu gene, near the site of switch recombination, were deleted from both the expressed and the nonexpressed chromosomes. Although mu genes were deleted from both chromosomes in the IgA+ cells, the sites of DNA recombination differed on the two chromosomes. On the expressed chromosome, Smu sequences were recombined with S alpha sequences, whereas on the nonexpressed chromosome, Smu sequences were recombined with S gamma 3 sequences.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6290869&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC369888/
dc.subjectAnimals
dc.subject*B-Lymphocytes
dc.subjectBase Sequence
dc.subjectClone Cells
dc.subjectDNA
dc.subjectDNA Restriction Enzymes
dc.subject*Gene Expression Regulation
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin Allotypes
dc.subjectImmunoglobulin Heavy Chains
dc.subjectImmunoglobulin M
dc.subjectImmunoglobulin alpha-Chains
dc.subjectImmunoglobulin mu-Chains
dc.subjectLymphoma
dc.subjectMice
dc.subjectNeoplasms, Experimental
dc.subjectReceptors, Antigen, B-Cell
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectWomen's Studies
dc.titleRearrangements and deletions of immunoglobulin heavy chain genes in the double-producing B cell lymphoma I.29
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biology
dc.source.volume2
dc.source.issue8
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/wfc_pp/243
dc.identifier.contextkey367704
html.description.abstract<p>The B cell lymphoma I.29 consists of a mixture of cells expressing membrane-bound immunoglobulin M (IgM) (lambda) and IgA (lambda) of identical idiotypes. Whereas most of the cells express either IgM or IgA alone, 1 to 5% of the cells in this tumor express IgM and IgA simultaneously within the cytoplasm and on the cell membrane (R. Sitia et al., J. Immunol. 127:1388-1394, 1981; R. Sitia, unpublished data). When IgM+ cells are purified from the lymphoma and passaged in mice or cultured, a portion of the cells convert to IgA+. These properties suggest that some cells of the I.29 lymphoma may undergo immunoglobulin heavy chain switching, although it is also possible that the mixed population was derived by a prior switching event in a clone of cells. We performed Southern blotting experiments on genomic DNAs isolated from populations of I.29 cells containing variable proportions of IgM+ and IgA+ cells and on a number of cell lines derived from the lymphoma. The results were consistent with the deletion model for heavy chain switching, as the IgM+ cells contained rearranged mu genes and alpha genes in the germ line configuration on both the expressed and nonexpressed heavy chain chromosomes, whereas the IgA+ cells had deleted both mu genes and contained one rearranged and one germ line alpha gene. In addition, segments of DNA located within the intervening sequence 5' to the mu gene, near the site of switch recombination, were deleted from both the expressed and the nonexpressed chromosomes. Although mu genes were deleted from both chromosomes in the IgA+ cells, the sites of DNA recombination differed on the two chromosomes. On the expressed chromosome, Smu sequences were recombined with S alpha sequences, whereas on the nonexpressed chromosome, Smu sequences were recombined with S gamma 3 sequences.</p>
dc.identifier.submissionpathwfc_pp/243
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages1002-13


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