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dc.contributor.authorShin, H. S.
dc.contributor.authorStavnezer, Janet
dc.contributor.authorArtzt, K.
dc.contributor.authorBennett, David A.
dc.date2022-08-11T08:11:04.000
dc.date.accessioned2022-08-23T17:31:22Z
dc.date.available2022-08-23T17:31:22Z
dc.date.issued1982-07-01
dc.date.submitted2007-09-14
dc.identifier.citation<p>Cell. 1982 Jul;29(3):969-76.</p>
dc.identifier.issn0092-8674 (Print)
dc.identifier.doi10.1016/0092-8674(82)90460-3
dc.identifier.pmid6295638
dc.identifier.urihttp://hdl.handle.net/20.500.14038/50717
dc.description.abstractWe investigated the genetic organization and evolutionary origin of t chromosomes of mice by examining the restriction fragment patterns of DNA from t haplotypes and normal chromosomes with cDNA probes to H-2 class I genes. On genomic DNA blots, the restriction fragments containing H-2-related sequences were highly variable among different inbred strains of mice, whereas they were very similar among different t haplotypes even when the t haplotypes carried serologically different H-2 haplotypes. These observations suggest that all t haplotypes have a common origin and are not products of independent mutational events. We also mapped the position of several restriction fragments characteristic of t DNA by using a battery of recombinant t haplotypes, defined with respect to their t-lethal factors and H-2 haplotypes. We thus show that restriction fragments containing H-2-related sequences map to the left of the H-2 class I genes in t chromosomes, a region in which the tw32 b-lethal factor also maps. The cloning of these fragments can be expected to provide an entry for the structural analysis of t DNA.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6295638&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttp://dx.doi.org/10.1016/0092-8674(82)90460-3
dc.subjectAnimals
dc.subjectChromosome Mapping
dc.subjectCloning, Molecular
dc.subjectDNA
dc.subjectDNA Restriction Enzymes
dc.subjectH-2 Antigens
dc.subjectMice
dc.subjectMice, Mutant Strains
dc.subjectNucleic Acid Hybridization
dc.subjectPolymorphism, Genetic
dc.subjectRecombination, Genetic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectWomen's Studies
dc.titleGenetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes
dc.typeJournal Article
dc.source.journaltitleCell
dc.source.volume29
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/wfc_pp/244
dc.identifier.contextkey367705
html.description.abstract<p>We investigated the genetic organization and evolutionary origin of t chromosomes of mice by examining the restriction fragment patterns of DNA from t haplotypes and normal chromosomes with cDNA probes to H-2 class I genes. On genomic DNA blots, the restriction fragments containing H-2-related sequences were highly variable among different inbred strains of mice, whereas they were very similar among different t haplotypes even when the t haplotypes carried serologically different H-2 haplotypes. These observations suggest that all t haplotypes have a common origin and are not products of independent mutational events. We also mapped the position of several restriction fragments characteristic of t DNA by using a battery of recombinant t haplotypes, defined with respect to their t-lethal factors and H-2 haplotypes. We thus show that restriction fragments containing H-2-related sequences map to the left of the H-2 class I genes in t chromosomes, a region in which the tw32 b-lethal factor also maps. The cloning of these fragments can be expected to provide an entry for the structural analysis of t DNA.</p>
dc.identifier.submissionpathwfc_pp/244
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages969-76


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