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    Membrane-bound and secreted IgA contain structurally different alpha-chains

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    Authors
    Sitia, R.
    Kikutani, H.
    Rubartelli, A.
    Bushkin, Y.
    Stavnezer, Janet
    Hammerling, U.
    UMass Chan Affiliations
    Department of Molecular Genetics and Microbiology
    Document Type
    Journal Article
    Publication Date
    1982-02-01
    Keywords
    Animals
    Antibody-Producing Cells
    B-Lymphocytes
    Cell Line
    Chemistry
    Electrophoresis, Polyacrylamide Gel
    Goats
    Immunoglobulin A
    *Immunoglobulin Heavy Chains
    *Immunoglobulin alpha-Chains
    Lymphoma
    Mice
    Mice, Inbred Strains
    Receptors, Antigen, B-Cell
    Tunicamycin
    Life Sciences
    Medicine and Health Sciences
    Women's Studies
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    Link to Full Text
    http://www.jimmunol.org/content/128/2/712.long
    Abstract
    Three different forms of alpha-chains are synthesized by BF0.3 and 615.2, two cloned cell lines derived from the murine B lymphoma 1.29. The three forms of alpha-chains differ in size, pI, cellular location, and rate of turnover. They were identified by means of lactoperoxidase-catalyzed radioiodination, internal 14C or 35S labeling, and immunofluorescence techniques as membrane-bound(alpha m), secreted (alpha s), and intracellular (alpha ic) proteins. Comparison of immunoglobulin products of the two lymphoma lines with those of a hybridoma cell line, Id 150, which secretes IgA of the 1.29 idiotype but lacks membrane IgA, confirmed the assignments of alpha m, alpha s, and alpha ic. Results of biosynthetic labeling of BF0.3, 615.2, and Id 150 in the presence and absence of tunicamycin suggest that the difference in m.w. and charge observed between alpha m and alpha s can be attributed to differences in primary amino acid structure rather than different degrees of glycosylation.
    Source
    J Immunol. 1982 Feb;128(2):712-6.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/50718
    PubMed ID
    6798121
    Related Resources
    Link to article in PubMed
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    UMass Chan Faculty and Researcher Publications

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