B-cell responses during primary and secondary dengue virus infections in humans
Gibbons, Robert V.
Libraty, Daniel H.
Rothman, Alan L.
UMass Chan AffiliationsDepartment of Medicine, Center for Infectious Diseases and Immunology
Document TypeJournal Article
Enzyme-Linked Immunosorbent Assay
Enzyme-Linked Immunospot Assay
Hemagglutination Inhibition Tests
Viral Envelope Proteins
Viral Nonstructural Proteins
Medicine and Health Sciences
MetadataShow full item record
AbstractLow-avidity serotype-cross-reactive antibodies are hypothesized to play a key role in triggering severe disease in patients with secondary dengue virus (DENV) infection. However, there is little systematic information about the frequency, avidity, and cross-reactivity of DENV-specific B cells in individuals experiencing primary instead of secondary infection. We compared DENV-specific B-cell responses in a cohort of Thai children with primary or secondary DENV infection. B cells specific for DENV precursor membrane protein, envelope (E) protein, and nonstructural protein 1 were detectable in immune peripheral blood mononuclear cells with the highest frequencies of DENV E-specific B cells detected in patients experiencing primary DENV-1 infections. DENV E-specific B cells were highly serotype-specific after primary DENV infections, whereas most E-specific B cells in patients with secondary infection were serotype-cross-reactive and secreted antibodies with higher avidity to heterologous DENV serotypes. Our data suggest that the minor populations of serotype-cross-reactive B cells generated by primary DENV infection are preferentially expanded during secondary DENV infection.
J Infect Dis. 2011 Nov 15;204(10):1514-22. Epub 2011 Sep 19. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/50986