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    IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding

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    MBC_IC97_3044.pdf
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    Authors
    Wirschell, Maureen
    Yang, Chun
    Yang, Pinfen
    Fox, Laura
    Yanagisawa, Haru-aki
    Kamiya, Ritsu
    Witman, George B.
    Porter, Mary E.
    Sale, Winfield S.
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    2009-07-08
    Keywords
    Algal Proteins
    Amino Acid Sequence
    Animals
    Axoneme
    Base Sequence
    Blotting, Western
    Chlamydomonas reinhardtii
    Cloning, Molecular
    Dyneins
    Immunoprecipitation
    Microtubules
    Molecular Sequence Data
    Mutation
    Protein Binding
    Protein Subunits
    Sequence Analysis, DNA
    Sequence Homology, Amino Acid
    Tubulin
    Cell Biology
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    Abstract
    Our goal is to understand the assembly and regulation of flagellar dyneins, particularly the Chlamydomonas inner arm dynein called I1 dynein. Here, we focus on the uncharacterized I1-dynein IC IC97. The IC97 gene encodes a novel IC without notable structural domains. IC97 shares homology with the murine lung adenoma susceptibility 1 (Las1) protein--a candidate tumor suppressor gene implicated in lung tumorigenesis. Multiple, independent biochemical assays determined that IC97 interacts with both alpha- and beta-tubulin subunits within the axoneme. I1-dynein assembly mutants suggest that IC97 interacts with both the IC138 and IC140 subunits within the I1-dynein motor complex and that IC97 is part of a regulatory complex that contains IC138. Microtubule sliding assays, using axonemes containing I1 dynein but devoid of IC97, show reduced microtubule sliding velocities that are not rescued by kinase inhibitors, revealing a critical role for IC97 in I1-dynein function and control of dynein-driven motility.
    Source
    Mol Biol Cell. 2009 Jul;20(13):3044-54. Epub 2009 May 6. Link to article on publisher's site
    DOI
    10.1091/mbc.E09-04-0276
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/51089
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1091/mbc.E09-04-0276
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