Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells
dc.contributor.author | Silberstein, Lev | |
dc.contributor.author | Sánchez, María-José | |
dc.contributor.author | Socolovsky, Merav | |
dc.contributor.author | Liu, Ying | |
dc.contributor.author | Hoffman, Gary | |
dc.contributor.author | Kinston, Sarah | |
dc.contributor.author | Piltz, Sandie | |
dc.contributor.author | Bowen, Mark | |
dc.contributor.author | Gambardella, Laure | |
dc.contributor.author | Green, Anthony R | |
dc.contributor.author | Göttgens, Berthold | |
dc.date.accessioned | 2022-10-20T15:28:57Z | |
dc.date.available | 2022-10-20T15:28:57Z | |
dc.date.issued | 2005-07-28 | |
dc.identifier.citation | Silberstein L, Sánchez MJ, Socolovsky M, Liu Y, Hoffman G, Kinston S, Piltz S, Bowen M, Gambardella L, Green AR, Göttgens B. Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells. Stem Cells. 2005 Oct;23(9):1378-88. doi: 10.1634/stemcells.2005-0090. Epub 2005 Jul 28. PMID: 16051983. | en_US |
dc.identifier.issn | 1066-5099 | |
dc.identifier.doi | 10.1634/stemcells.2005-0090 | en_US |
dc.identifier.pmid | 16051983 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/51185 | |
dc.description.abstract | Appropriate transcriptional regulation is critical for the biological functions of many key regulatory genes, including the stem cell leukemia (SCL) gene. As part of a systematic dissection of SCL transcriptional regulation, we have previously identified a 5,245-bp SCL +18/19 enhancer that targeted embryonic endothelium together with embryonic and adult hematopoietic progenitors and stem cells (HSCs). This enhancer is proving to be a powerful tool for manipulating hematopoietic progenitors and stem cells, but the design and interpretation of such transgenic studies require a detailed understanding of enhancer activity in vivo. In this study, we demonstrate that the +18/19 enhancer is active in mast cells, megakaryocytes, and adult endothelium. A 644-bp +19 core enhancer exhibited similar temporal and spatial activity to the 5,245-bp +18/19 fragment both during development and in adult mice. Unlike the +18/19 enhancer, the +19 core enhancer was only active in adult mice when linked to the eukaryotic reporter gene human placental alkaline phosphatase. Activity of a single core enhancer in HSCs, endothelium, mast cells, and megakaryocytes suggests possible overlaps in their respective transcriptional programs. Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Stem Cells | en_US |
dc.relation.url | https://doi.org/10.1634/stemcells.2005-0090 | en_US |
dc.subject | Hematopoietic stem cell | en_US |
dc.subject | Transcription factor SCL/tal‐1 | en_US |
dc.subject | Transcriptional regulation | en_US |
dc.subject | Experimental models | en_US |
dc.title | Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells | en_US |
dc.type | Journal Article | en_US |
dc.source.journaltitle | Stem cells (Dayton, Ohio) | |
dc.source.volume | 23 | |
dc.source.issue | 9 | |
dc.source.beginpage | 1378 | |
dc.source.endpage | 88 | |
dc.source.country | United Kingdom | |
dc.source.country | England | |
dc.identifier.journal | Stem cells (Dayton, Ohio) | |
dc.contributor.department | Molecular, Cell and Cancer Biology | en_US |
dc.contributor.department | Pediatrics | en_US |