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dc.contributor.authorSilberstein, Lev
dc.contributor.authorSánchez, María-José
dc.contributor.authorSocolovsky, Merav
dc.contributor.authorLiu, Ying
dc.contributor.authorHoffman, Gary
dc.contributor.authorKinston, Sarah
dc.contributor.authorPiltz, Sandie
dc.contributor.authorBowen, Mark
dc.contributor.authorGambardella, Laure
dc.contributor.authorGreen, Anthony R
dc.contributor.authorGöttgens, Berthold
dc.date.accessioned2022-10-20T15:28:57Z
dc.date.available2022-10-20T15:28:57Z
dc.date.issued2005-07-28
dc.identifier.citationSilberstein L, Sánchez MJ, Socolovsky M, Liu Y, Hoffman G, Kinston S, Piltz S, Bowen M, Gambardella L, Green AR, Göttgens B. Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells. Stem Cells. 2005 Oct;23(9):1378-88. doi: 10.1634/stemcells.2005-0090. Epub 2005 Jul 28. PMID: 16051983.en_US
dc.identifier.issn1066-5099
dc.identifier.doi10.1634/stemcells.2005-0090en_US
dc.identifier.pmid16051983
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51185
dc.description.abstractAppropriate transcriptional regulation is critical for the biological functions of many key regulatory genes, including the stem cell leukemia (SCL) gene. As part of a systematic dissection of SCL transcriptional regulation, we have previously identified a 5,245-bp SCL +18/19 enhancer that targeted embryonic endothelium together with embryonic and adult hematopoietic progenitors and stem cells (HSCs). This enhancer is proving to be a powerful tool for manipulating hematopoietic progenitors and stem cells, but the design and interpretation of such transgenic studies require a detailed understanding of enhancer activity in vivo. In this study, we demonstrate that the +18/19 enhancer is active in mast cells, megakaryocytes, and adult endothelium. A 644-bp +19 core enhancer exhibited similar temporal and spatial activity to the 5,245-bp +18/19 fragment both during development and in adult mice. Unlike the +18/19 enhancer, the +19 core enhancer was only active in adult mice when linked to the eukaryotic reporter gene human placental alkaline phosphatase. Activity of a single core enhancer in HSCs, endothelium, mast cells, and megakaryocytes suggests possible overlaps in their respective transcriptional programs. Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus.en_US
dc.language.isoenen_US
dc.relation.ispartofStem Cellsen_US
dc.relation.urlhttps://doi.org/10.1634/stemcells.2005-0090en_US
dc.subjectHematopoietic stem cellen_US
dc.subjectTranscription factor SCL/tal‐1en_US
dc.subjectTranscriptional regulationen_US
dc.subjectExperimental modelsen_US
dc.titleTransgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cellsen_US
dc.typeJournal Articleen_US
dc.source.journaltitleStem cells (Dayton, Ohio)
dc.source.volume23
dc.source.issue9
dc.source.beginpage1378
dc.source.endpage88
dc.source.countryUnited Kingdom
dc.source.countryEngland
dc.identifier.journalStem cells (Dayton, Ohio)
dc.contributor.departmentMolecular, Cell and Cancer Biologyen_US
dc.contributor.departmentPediatricsen_US


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