Shear stress activates nociceptors to drive Drosophila mechanical nociception
Ruppell, Kendra Takle
Student AuthorsKendra Takle Ruppell
UMass Chan AffiliationsMorningside Graduate School of Biomedical Sciences
Yang Xiang Lab
Document TypeJournal Article
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AbstractMechanical nociception is essential for animal survival. However, the forces involved in nociceptor activation and the underlying mechanotransduction mechanisms remain elusive. Here, we address these problems by investigating nocifensive behavior in Drosophila larvae. We show that strong poking stimulates nociceptors with a mixture of forces including shear stress and stretch. Unexpectedly, nociceptors are selectively activated by shear stress, but not stretch. Both the shear stress responses of nociceptors and nocifensive behavior require transient receptor potential A1 (TrpA1), which is specifically expressed in nociceptors. We further demonstrate that expression of mammalian or Drosophila TrpA1 in heterologous cells confers responses to shear stress but not stretch. Finally, shear stress activates TrpA1 in a membrane-delimited manner, through modulation of membrane fluidity. Together, our study reveals TrpA1 as an evolutionarily conserved mechanosensitive channel specifically activated by shear stress and suggests a critical role of shear stress in activating nociceptors to drive mechanical nociception.
SourceGong J, Chen J, Gu P, Shang Y, Ruppell KT, Yang Y, Wang F, Wen Q, Xiang Y. Shear stress activates nociceptors to drive Drosophila mechanical nociception. Neuron. 2022 Sep 2:S0896-6273(22)00747-4. doi: 10.1016/j.neuron.2022.08.015. Epub ahead of print. PMID: 36087585.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/51226
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