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dc.contributor.authorElferich, Johannes
dc.contributor.authorSchiroli, Giulia
dc.contributor.authorScadden, David T
dc.contributor.authorGrigorieff, Nikolaus
dc.date.accessioned2023-01-30T20:17:39Z
dc.date.available2023-01-30T20:17:39Z
dc.date.issued2022-11-16
dc.identifier.citationElferich J, Schiroli G, Scadden DT, Grigorieff N. Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sections. Elife. 2022 Nov 16;11:e80980. doi: 10.7554/eLife.80980. PMID: 36382886; PMCID: PMC9711527.en_US
dc.identifier.eissn2050-084X
dc.identifier.doi10.7554/eLife.80980en_US
dc.identifier.pmid36382886
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51604
dc.description.abstractA major goal of biological imaging is localization of biomolecules inside a cell. Fluorescence microscopy can localize biomolecules inside whole cells and tissues, but its ability to count biomolecules and accuracy of the spatial coordinates is limited by the wavelength of visible light. Cryo-electron microscopy (cryo-EM) provides highly accurate position and orientation information of biomolecules but is often confined to small fields of view inside a cell, limiting biological context. In this study, we use a new data-acquisition scheme called Defocus-Corrected Large-Area cryo-EM (DeCo-LACE) to collect high-resolution images of entire sections (100- to 250-nm-thick lamellae) of neutrophil-like mouse cells, representing 1-2% of the total cellular volume. We use 2D template matching (2DTM) to determine localization and orientation of the large ribosomal subunit in these sections. These data provide maps of ribosomes across entire sections of mammalian cells. This high-throughput cryo-EM data collection approach together with 2DTM will advance visual proteomics and provide biological insight that cannot be obtained by other methods.en_US
dc.language.isoenen_US
dc.relation.ispartofElifeen_US
dc.relation.urlhttps://doi.org/10.7554/elife.80980en_US
dc.rightsCopyright Elferich et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcell biologyen_US
dc.subjectcryo-EMen_US
dc.subjectmolecular biophysicsen_US
dc.subjectmouseen_US
dc.subjectneutrophilen_US
dc.subjectribosomeen_US
dc.subjectstructural biologyen_US
dc.titleDefocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sectionsen_US
dc.typeJournal Articleen_US
dc.source.journaltitleeLife
dc.source.volume11
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryEngland
dc.identifier.journaleLife
refterms.dateFOA2023-01-30T20:17:40Z
dc.contributor.departmentRNA Therapeutics Instituteen_US


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Copyright Elferich et al. This
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Except where otherwise noted, this item's license is described as Copyright Elferich et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.