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dc.contributor.authorSchoenborn, Alexi A
dc.contributor.authorYannarell, Sarah M
dc.contributor.authorWallace, E Diane
dc.contributor.authorClapper, Haley
dc.contributor.authorWeinstein, Ilon C
dc.contributor.authorShank, Elizabeth A
dc.date.accessioned2023-02-09T19:29:26Z
dc.date.available2023-02-09T19:29:26Z
dc.date.issued2021-08-30
dc.identifier.citationSchoenborn AA, Yannarell SM, Wallace ED, Clapper H, Weinstein IC, Shank EA. Defining the Expression, Production, and Signaling Roles of Specialized Metabolites during Bacillus subtilis Differentiation. J Bacteriol. 2021 Oct 25;203(22):e0033721. doi: 10.1128/JB.00337-21. Epub 2021 Aug 30. PMID: 34460312; PMCID: PMC8544424.en_US
dc.identifier.eissn1098-5530
dc.identifier.doi10.1128/JB.00337-21en_US
dc.identifier.pmid34460312
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51657
dc.description.abstractBacterial specialized (or secondary) metabolites are structurally diverse molecules that mediate intra- and interspecies interactions by altering growth and cellular physiology and differentiation. Bacillus subtilis, a Gram-positive model bacterium commonly used to study biofilm formation and sporulation, has the capacity to produce more than 10 specialized metabolites. Some of these B. subtilis specialized metabolites have been investigated for their role in facilitating cellular differentiation, but only rarely has the behavior of multiple metabolites been simultaneously investigated. In this study, we explored the interconnectivity of differentiation (biofilm and sporulation) and specialized metabolites in B. subtilis. Specifically, we interrogated how development influences specialized metabolites and vice versa. Using the sporulation-inducing medium DSM, we found that the majority of the specialized metabolites examined are expressed and produced during biofilm formation and sporulation. Additionally, we found that six of these metabolites (surfactin, ComX, bacillibactin, bacilysin, subtilosin A, and plipastatin) are necessary signaling molecules for proper progression of B. subtilis differentiation. This study further supports the growing body of work demonstrating that specialized metabolites have essential physiological functions as cell-cell communication signals in bacteria. IMPORTANCE Bacterially produced specialized metabolites are frequently studied for their potential use as antibiotics and antifungals. However, a growing body of work has suggested that the antagonistic potential of specialized metabolites is not their only function. Here, using Bacillus subtilis as our model bacterium, we demonstrated that developmental processes such as biofilm formation and sporulation are tightly linked to specialized metabolite gene expression and production. Additionally, under our differentiation-inducing conditions, six out of the nine specialized metabolites investigated behave as intraspecific signals that impact B. subtilis physiology and influence biofilm formation and sporulation. Our work supports the viewpoint that specialized metabolites have a clear role as cell-cell signaling molecules within differentiated populations of bacteria.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Bacteriologyen_US
dc.relation.urlhttps://doi.org/10.1128/jb.00337-21en_US
dc.subjectBacillus subtilisen_US
dc.subjectbacterial developmenten_US
dc.subjectbioactive moleculesen_US
dc.subjectbiofilm formationen_US
dc.subjectcell-cell signalingen_US
dc.subjectsecondary metabolitesen_US
dc.subjectspecialized metabolitesen_US
dc.subjectsporulationen_US
dc.titleDefining the Expression, Production, and Signaling Roles of Specialized Metabolites during Bacillus subtilis Differentiationen_US
dc.typeJournal Articleen_US
dc.source.journaltitleJournal of bacteriology
dc.source.volume203
dc.source.issue22
dc.source.beginpagee0033721
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalJournal of bacteriology
dc.contributor.departmentSystems Biologyen_US


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