Systems-level effects of allosteric perturbations to a model molecular switch
Authors
Perica, TinaMathy, Christopher J P
Xu, Jiewei
Jang, Gwendolyn Μ
Zhang, Yang
Kaake, Robyn
Ollikainen, Noah
Braberg, Hannes
Swaney, Danielle L
Lambright, David G
Kelly, Mark J S
Krogan, Nevan J
Kortemme, Tanja
Document Type
Journal ArticlePublication Date
2021-10-13Keywords
GTP-binding protein regulatorsMolecular conformation
Networks and systems biology
Proteomic analysis
Regulatory networks
Metadata
Show full item recordAbstract
Molecular switch proteins whose cycling between states is controlled by opposing regulators1,2 are central to biological signal transduction. As switch proteins function within highly connected interaction networks3, the fundamental question arises of how functional specificity is achieved when different processes share common regulators. Here we show that functional specificity of the small GTPase switch protein Gsp1 in Saccharomyces cerevisiae (the homologue of the human protein RAN)4 is linked to differential sensitivity of biological processes to different kinetics of the Gsp1 (RAN) switch cycle. We make 55 targeted point mutations to individual protein interaction interfaces of Gsp1 (RAN) and show through quantitative genetic5 and physical interaction mapping that Gsp1 (RAN) interface perturbations have widespread cellular consequences. Contrary to expectation, the cellular effects of the interface mutations group by their biophysical effects on kinetic parameters of the GTPase switch cycle and not by the targeted interfaces. Instead, we show that interface mutations allosterically tune the GTPase cycle kinetics. These results suggest a model in which protein partner binding, or post-translational modifications at distal sites, could act as allosteric regulators of GTPase switching. Similar mechanisms may underlie regulation by other GTPases, and other biological switches. Furthermore, our integrative platform to determine the quantitative consequences of molecular perturbations may help to explain the effects of disease mutations that target central molecular switches.Source
Perica T, Mathy CJP, Xu J, Jang GΜ, Zhang Y, Kaake R, Ollikainen N, Braberg H, Swaney DL, Lambright DG, Kelly MJS, Krogan NJ, Kortemme T. Systems-level effects of allosteric perturbations to a model molecular switch. Nature. 2021 Nov;599(7883):152-157. doi: 10.1038/s41586-021-03982-6. Epub 2021 Oct 13. PMID: 34646016; PMCID: PMC8571063.DOI
10.1038/s41586-021-03982-6Permanent Link to this Item
http://hdl.handle.net/20.500.14038/51671PubMed ID
34646016Rights
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.ae974a485f413a2113503eed53cd6c53
10.1038/s41586-021-03982-6