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dc.contributor.authorGuharajan, Sunil
dc.contributor.authorChhabra, Shivani
dc.contributor.authorParisutham, Vinuselvi
dc.contributor.authorBrewster, Robert C
dc.date.accessioned2023-02-16T21:21:00Z
dc.date.available2023-02-16T21:21:00Z
dc.date.issued2021-11-09
dc.identifier.citationGuharajan S, Chhabra S, Parisutham V, Brewster RC. Quantifying the regulatory role of individual transcription factors in Escherichia coli. Cell Rep. 2021 Nov 9;37(6):109952. doi: 10.1016/j.celrep.2021.109952. PMID: 34758318; PMCID: PMC8667592.en_US
dc.identifier.eissn2211-1247
dc.identifier.doi10.1016/j.celrep.2021.109952en_US
dc.identifier.pmid34758318
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51710
dc.description.abstractGene regulation often results from the action of multiple transcription factors (TFs) acting at a promoter, obscuring the individual regulatory effect of each TF on RNA polymerase (RNAP). Here we measure the fundamental regulatory interactions of TFs in E. coli by designing synthetic target genes that isolate individual TFs' regulatory effects. Using a thermodynamic model, each TF's regulatory interactions are decoupled from TF occupancy and interpreted as acting through (de)stabilization of RNAP and (de)acceleration of transcription initiation. We find that the contribution of each mechanism depends on TF identity and binding location; regulation immediately downstream of the promoter is insensitive to TF identity, but the same TFs regulate by distinct mechanisms upstream of the promoter. These two mechanisms are uncoupled and can act coherently, to reinforce the observed regulatory role (activation/repression), or incoherently, wherein the TF regulates two distinct steps with opposing effects.en_US
dc.language.isoenen_US
dc.relationThis article is based on a previously available preprint in bioRxiv, https://doi.org/10.1101/2021.01.04.425191.
dc.relation.ispartofCell Reportsen_US
dc.relation.urlhttps://doi.org/10.1016/j.celrep.2021.109952en_US
dc.rightsCopyright 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).; Attribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectgene regulationen_US
dc.subjectquantitative modelingen_US
dc.subjectsynthetic biologyen_US
dc.subjectsystems biologyen_US
dc.subjecttranscription factoren_US
dc.titleQuantifying the regulatory role of individual transcription factors in Escherichia colien_US
dc.typeJournal Articleen_US
dc.source.journaltitleCell reports
dc.source.volume37
dc.source.issue6
dc.source.beginpage109952
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalCell reports
refterms.dateFOA2023-02-16T21:21:01Z
dc.contributor.departmentMicrobiology and Physiological Systemsen_US
dc.contributor.departmentMorningside Graduate School of Biomedical Sciencesen_US
dc.contributor.departmentSystems Biologyen_US


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Copyright 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).; Attribution 4.0 International
Except where otherwise noted, this item's license is described as Copyright 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).; Attribution 4.0 International