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dc.contributor.authorHe, Feng
dc.contributor.authorWu, Chan
dc.contributor.authorJacobson, Allan
dc.date.accessioned2023-03-03T16:15:14Z
dc.date.available2023-03-03T16:15:14Z
dc.date.issued2022-05-23
dc.identifier.citationHe F, Wu C, Jacobson A. Dcp2 C-terminal cis-binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexes. Elife. 2022 May 23;11:e74410. doi: 10.7554/eLife.74410. PMID: 35604319; PMCID: PMC9170289.en_US
dc.identifier.eissn2050-084X
dc.identifier.doi10.7554/eLife.74410en_US
dc.identifier.pmid35604319
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51740
dc.description.abstractA single Dcp1-Dcp2 decapping enzyme targets diverse classes of yeast mRNAs for decapping-dependent 5' to 3' decay, but the molecular mechanisms controlling mRNA selectivity by the enzyme remain elusive. Through extensive genetic analyses we reveal that Dcp2 C-terminal domain cis-regulatory elements control decapping enzyme target specificity by orchestrating formation of distinct decapping complexes. Two Upf1-binding motifs direct the decapping enzyme to nonsense-mediated mRNA decay substrates, a single Edc3-binding motif targets both Edc3 and Dhh1 substrates, and Pat1-binding leucine-rich motifs target Edc3 and Dhh1 substrates under selective conditions. Although it functions as a unique targeting component of specific complexes, Edc3 is a common component of multiple complexes. Scd6 and Xrn1 also have specific binding sites on Dcp2, allowing them to be directly recruited to decapping complexes. Collectively, our results demonstrate that Upf1, Edc3, Scd6, and Pat1 function as regulatory subunits of the holo-decapping enzyme, controlling both its substrate specificity and enzymatic activation.en_US
dc.language.isoenen_US
dc.relationThis article is based on a previously available preprint in bioRxiv, https://doi.org/10.1101/2021.10.01.462794.en_US
dc.relation.ispartofeLifeen_US
dc.relation.urlhttps://doi.org/10.7554/elife.74410en_US
dc.rightsCopyright He et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.; Attribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDcp2en_US
dc.subjectEdc3en_US
dc.subjectNMDen_US
dc.subjectPat1en_US
dc.subjectS. cerevisiaeen_US
dc.subjectUpf1en_US
dc.subjectXrn1en_US
dc.subjectchromosomesen_US
dc.subjectgene expressionen_US
dc.subjectgeneticsen_US
dc.subjectgenomicsen_US
dc.titleDcp2 C-terminal -binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexesen_US
dc.typeJournal Articleen_US
dc.source.journaltitleeLife
dc.source.volume11
dc.source.countryUnited States
dc.source.countryEngland
dc.identifier.journaleLife
refterms.dateFOA2023-03-03T16:15:15Z
dc.contributor.departmentMicrobiology and Physiological Systemsen_US


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Copyright He et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.; Attribution 4.0 International
Except where otherwise noted, this item's license is described as Copyright He et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.; Attribution 4.0 International