Show simple item record

dc.contributor.authorRose, Rebecca
dc.contributor.authorGonzalez-Perez, Maria Paz
dc.contributor.authorNolan, David
dc.contributor.authorGanta, Krishna Kumar
dc.contributor.authorLaFleur, Tessa
dc.contributor.authorCross, Sissy
dc.contributor.authorBrody, Robin
dc.contributor.authorLamers, Susanna L
dc.contributor.authorLuzuriaga, Katherine
dc.date.accessioned2023-03-09T21:03:08Z
dc.date.available2023-03-09T21:03:08Z
dc.date.issued2022-09-29
dc.identifier.citationRose R, Gonzalez-Perez MP, Nolan D, Ganta KK, LaFleur T, Cross S, Brody R, Lamers SL, Luzuriaga K. Distinct HIV-1 Population Structure across Meningeal and Peripheral T Cells and Macrophage Lineage Cells. Microbiol Spectr. 2022 Oct 26;10(5):e0250822. doi: 10.1128/spectrum.02508-22. Epub 2022 Sep 29. PMID: 36173332; PMCID: PMC9602438.en_US
dc.identifier.eissn2165-0497
dc.identifier.doi10.1128/spectrum.02508-22en_US
dc.identifier.pmid36173332
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51782
dc.description.abstractHIV-1 sequence population structure among brain and nonbrain cellular compartments is incompletely understood. Here, we compared proviral pol and env high-quality consensus single-molecule real-time (SMRT) sequences derived from CD3+ T cells and CD14+ macrophage lineage cells from meningeal or peripheral (spleen, blood) tissues obtained at autopsy from two individuals with viral suppression on antiretroviral therapy (ART). Phylogenetic analyses showed strong evidence of population structure between CD3+ and CD14+ virus populations. Distinct env variable-region characteristics were also found between CD3+ and CD14+ viruses. Furthermore, shared macrophage-tropic amino acid residues (env) and drug resistance mutations (pol) between meningeal and peripheral virus populations were consistent with the meninges playing a role in viral gene flow across the blood-brain barrier. Overall, our results point toward potential functional differences among meningeal and peripheral CD3+ and CD14+ virus populations and a complex evolutionary history driven by distinct selection pressures and/or viral gene flow. IMPORTANCE Different cell types and/or tissues may serve as a reservoir for HIV-1 during ART-induced viral suppression. We compared proviral pol and env sequences from CD3+ T cells and CD14+ macrophage lineage cells from brain and nonbrain tissues from two virally suppressed individuals. We found strong evidence of viral population structure among cells/tissues, which may result from distinct selective pressures across cell types and anatomic sites.en_US
dc.language.isoenen_US
dc.relation.ispartofMicrobiology Spectrumen_US
dc.relation.urlhttps://doi.org/10.1128/spectrum.02508-22en_US
dc.rightsCopyright © 2022 Rose et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHIV/AIDSen_US
dc.subjectcompartmentalizationen_US
dc.subjectevolutionen_US
dc.subjectphylogeneticsen_US
dc.subjecttropismen_US
dc.subjectUMCCTS fundingen_US
dc.titleDistinct HIV-1 Population Structure across Meningeal and Peripheral T Cells and Macrophage Lineage Cellsen_US
dc.typeJournal Articleen_US
dc.source.journaltitleMicrobiology spectrum
dc.source.volume10
dc.source.issue5
dc.source.beginpagee0250822
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalMicrobiology spectrum
refterms.dateFOA2023-03-09T21:03:09Z
dc.contributor.departmentProgram in Molecular Medicineen_US


Files in this item

Thumbnail
Name:
spectrum.02508-22.pdf
Size:
677.4Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Copyright © 2022 Rose et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Except where otherwise noted, this item's license is described as Copyright © 2022 Rose et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.