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dc.contributor.authorZhang, Qian
dc.contributor.authorHuang, Zhen
dc.contributor.authorZuo, Huanyan
dc.contributor.authorLin, Yuxiu
dc.contributor.authorXiao, Yao
dc.contributor.authorYan, Yanan
dc.contributor.authorCui, Yu
dc.contributor.authorLin, Chujiao
dc.contributor.authorPei, Fei
dc.contributor.authorChen, Zhi
dc.contributor.authorLiu, Huan
dc.date.accessioned2023-03-14T15:44:52Z
dc.date.available2023-03-14T15:44:52Z
dc.date.issued2021-11-25
dc.identifier.citationZhang Q, Huang Z, Zuo H, Lin Y, Xiao Y, Yan Y, Cui Y, Lin C, Pei F, Chen Z, Liu H. Chromatin Accessibility Predetermines Odontoblast Terminal Differentiation. Front Cell Dev Biol. 2021 Nov 25;9:769193. doi: 10.3389/fcell.2021.769193. PMID: 34901015; PMCID: PMC8655119.en_US
dc.identifier.issn2296-634X
dc.identifier.doi10.3389/fcell.2021.769193en_US
dc.identifier.pmid34901015
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51806
dc.description.abstractEmbryonic development and stem cell differentiation are orchestrated by changes in sequential binding of regulatory transcriptional factors to their motifs. These processes are invariably accompanied by the alternations in chromatin accessibility, conformation, and histone modification. Odontoblast lineage originates from cranial neural crest cells and is crucial in dentinogenesis. Our previous work revealed several transcription factors (TFs) that promote odontoblast differentiation. However, it remains elusive as to whether chromatin accessibility affects odontoblast terminal differentiation. Herein, integration of single-cell RNA-seq and bulk RNA-seq revealed that in vitro odontoblast differentiation using dental papilla cells at E18.5 was comparable to the crown odontoblast differentiation trajectory of OC (osteocalcin)-positive odontogenic lineage. Before in vitro odontoblast differentiation, ATAC-seq and H3K27Ac CUT and Tag experiments demonstrated high accessibility of chromatin regions adjacent to genes associated with odontogenic potential. However, following odontoblastic induction, regions near mineralization-related genes became accessible. Integration of RNA-seq and ATAC-seq results further revealed that the expression levels of these genes were correlated with the accessibility of nearby chromatin. Time-course ATAC-seq experiments further demonstrated that odontoblast terminal differentiation was correlated with the occupation of the basic region/leucine zipper motif (bZIP) TF family, whereby we validated the positive role of ATF5 in vitro. Collectively, this study reports a global mapping of open chromatin regulatory elements during dentinogenesis and illustrates how these regions are regulated via dynamic binding of different TF families, resulting in odontoblast terminal differentiation. The findings also shed light on understanding the genetic regulation of dentin regeneration using dental mesenchymal stem cells.en_US
dc.language.isoenen_US
dc.relation.ispartofFrontiers in Cell and Developmental Biologyen_US
dc.relation.urlhttps://doi.org/10.3389/fcell.2021.769193en_US
dc.rightsCopyright © 2021 Zhang, Huang, Zuo, Lin, Xiao, Yan, Cui, Lin, Pei, Chen and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectH3K27acen_US
dc.subjectRNA-seqen_US
dc.subjectchromatin immunoprecipitation sequencingen_US
dc.subjectdental mesenchymal stem cellsen_US
dc.subjectepigeneticsen_US
dc.subjectodontogenesisen_US
dc.subjecttooth developmenten_US
dc.subjecttranscription factorsen_US
dc.titleChromatin Accessibility Predetermines Odontoblast Terminal Differentiationen_US
dc.typeJournal Articleen_US
dc.source.journaltitleFrontiers in cell and developmental biology
dc.source.volume9
dc.source.beginpage769193
dc.source.endpage
dc.source.countrySwitzerland
dc.identifier.journalFrontiers in cell and developmental biology
refterms.dateFOA2023-03-14T15:44:52Z
dc.contributor.departmentMedicineen_US


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Copyright © 2021 Zhang, Huang, Zuo, Lin, Xiao, Yan, Cui, Lin, Pei, Chen and Liu.
This is an open-access article distributed under the terms of the Creative
Commons Attribution License (CC BY). The use, distribution or
reproduction in other forums is permitted, provided the original author(s)
and the copyright owner(s) are credited and that the original publication in this
journal is cited, in accordance with accepted academic practice. No use,
distribution or reproduction is permitted which does not comply with
these terms.
Except where otherwise noted, this item's license is described as Copyright © 2021 Zhang, Huang, Zuo, Lin, Xiao, Yan, Cui, Lin, Pei, Chen and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.