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dc.contributor.authorQu, Mingzi
dc.contributor.authorLu, Ping
dc.contributor.authorBellve, Karl
dc.contributor.authorLifshitz, Lawrence M
dc.contributor.authorZhuGe, Ronghua
dc.date.accessioned2023-03-16T15:18:01Z
dc.date.available2023-03-16T15:18:01Z
dc.date.issued2021-11-04
dc.identifier.citationQu M, Lu P, Bellve K, Lifshitz LM, ZhuGe R. Mode Switch of Ca2 + Oscillation-Mediated Uterine Peristalsis and Associated Embryo Implantation Impairments in Mouse Adenomyosis. Front Physiol. 2021 Nov 4;12:744745. doi: 10.3389/fphys.2021.744745. PMID: 34803733; PMCID: PMC8599363.en_US
dc.identifier.issn1664-042X
dc.identifier.doi10.3389/fphys.2021.744745en_US
dc.identifier.pmid34803733
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51829
dc.description.abstractAdenomyosis is a debilitating gynecological disease of the uterus with no medicinal cure. The tissue injury and repair hypothesis for adenomyosis suggests that uterine hyperperistalsis or dysperistalsis plays a pivotal role in establishing adenomyotic lesions. However, specific impairments in uterine peristalsis and the underlying cellular signals for these changes in adenomyosis remain elusive. Here, we report a precision-cut uterine slice preparation that preserves in vivo uterine architecture and generates peristalsis similar to that seen in the whole uterus. We found that uterine peristalsis in neonatal mice at day 14 and adult mice at day 55 presents as bursts with multiple peaks induced by intracellular Ca2+ oscillations. Using a mouse model of adenomyosis induced by tamoxifen, a selective estrogen receptor modulator, we discovered that uterine peristalsis and Ca2+ oscillations from adenomyotic uteri on days 14 and 55 become spikes (single peaks) with smaller amplitudes. The peak frequency of Ca2+ oscillations or peristalsis does not show a difference between control and adenomyotic mice. However, both the estimated force generated by uterine peristalsis and the total Ca2+ raised by Ca2+ oscillations are smaller in uteri from adenomyotic mice. Uteri from adenomyotic mice on day 14, but not on day 55, exhibit hyperresponsiveness to oxytocin. Embryo implantations are decreased in adenomyotic adult mice. Our results reveal a mode switch from bursts to spikes (rather than an increased peak frequency) of uterine Ca2+ oscillations and peristalsis and concurrent hyperresponsiveness to oxytocin in the neonatal stage are two characteristics of adenomyosis. These characteristics may contribute to embryo implantation impairments and decreased fertility in adenomyosis.en_US
dc.language.isoenen_US
dc.relation.ispartofFrontiers in Physiologyen_US
dc.relation.urlhttps://doi.org/10.3389/fphys.2021.744745en_US
dc.rightsCopyright © 2021 Qu, Lu, Bellve, Lifshitz and ZhuGe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCa2+ oscillationsen_US
dc.subjectadenomyosisen_US
dc.subjectembryo implantationen_US
dc.subjectmyometriumen_US
dc.subjectuterine peristalsisen_US
dc.titleMode Switch of Ca Oscillation-Mediated Uterine Peristalsis and Associated Embryo Implantation Impairments in Mouse Adenomyosisen_US
dc.typeJournal Articleen_US
dc.source.journaltitleFrontiers in physiology
dc.source.volume12
dc.source.beginpage744745
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countrySwitzerland
dc.identifier.journalFrontiers in physiology
refterms.dateFOA2023-03-16T15:18:02Z
dc.contributor.departmentMicrobiology and Physiological Systemsen_US
dc.contributor.departmentProgram in Molecular Medicineen_US


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Copyright © 2021 Qu, Lu, Bellve, Lifshitz and ZhuGe. This is an open-access article
distributed under the terms of the Creative Commons Attribution License (CC BY).
The use, distribution or reproduction in other forums is permitted, provided the
original author(s) and the copyright owner(s) are credited and that the original
publication in this journal is cited, in accordance with accepted academic practice. No
use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as Copyright © 2021 Qu, Lu, Bellve, Lifshitz and ZhuGe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.