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dc.contributor.authorBharadwaj, Ravi
dc.contributor.authorKushwaha, Tushar
dc.contributor.authorAhmad, Azhar
dc.contributor.authorInampudi, Krishna K
dc.contributor.authorNozaki, Tomoyoshi
dc.contributor.authorSomlata
dc.date.accessioned2023-03-24T16:49:13Z
dc.date.available2023-03-24T16:49:13Z
dc.date.issued2021-11-22
dc.identifier.citationBharadwaj R, Kushwaha T, Ahmad A, Inampudi KK, Nozaki T, Somlata. An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1. PLoS Pathog. 2021 Nov 22;17(11):e1010030. doi: 10.1371/journal.ppat.1010030. PMID: 34807955; PMCID: PMC8648123.en_US
dc.identifier.eissn1553-7374
dc.identifier.doi10.1371/journal.ppat.1010030en_US
dc.identifier.pmid34807955
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51885
dc.description.abstractThe parasite Entamoeba histolytica is the etiological agent of amoebiasis, a major cause of morbidity and mortality due to parasitic diseases in developing countries. Phagocytosis is an essential mode of obtaining nutrition and has been associated with the virulence behaviour of E. histolytica. Signalling pathways involved in activation of cytoskeletal dynamics required for phagocytosis remains to be elucidated in this parasite. Our group has been studying initiation of phagocytosis and formation of phagosomes in E. histolytica and have described some of the molecules that play key roles in the process. Here we showed the involvement of non-Dbl Rho Guanine Nucleotide Exchange Factor, EhGEF in regulation of amoebic phagocytosis by regulating activation of EhRho1. EhGEF was found in the phagocytic cups during the progression of cups, until closure of phagosomes, but not in the phagosomes themselves. Our observation from imaging, pull down experiments and down regulating expression of different molecules suggest that EhGEF interacts with EhRho1 and it is required during initiation of phagocytosis and phagosome formation. Also, biophysical, and computational analysis reveals that EhGEF mediates GTP exchange on EhRho1 via an unconventional pathway. In conclusion, we describe a non-Dbl EhGEF of EhRho1 which is involved in endocytic processes of E. histolytica.en_US
dc.language.isoenen_US
dc.relation.ispartofPLoS Pathogensen_US
dc.relation.urlhttps://doi.org/10.1371/journal.ppat.1010030en_US
dc.rightsCopyright: © 2021 Bharadwaj et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPhagocytosisen_US
dc.subjectEntamoeba histolyticaen_US
dc.subjectCell membranesen_US
dc.subjectPhagosomesen_US
dc.subjectTetracyclinesen_US
dc.subjectTrophozoitesen_US
dc.subjectGuanine nucleotide exchange factorsen_US
dc.subjectGuanosine triphosphataseen_US
dc.titleAn atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1en_US
dc.typeJournal Articleen_US
dc.source.journaltitlePLoS pathogens
dc.source.volume17
dc.source.issue11
dc.source.beginpagee1010030
dc.source.endpage
dc.source.countryUnited States
dc.identifier.journalPLoS pathogens
refterms.dateFOA2023-03-24T16:49:13Z
dc.contributor.departmentMedicineen_US


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Copyright: © 2021 Bharadwaj et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
Except where otherwise noted, this item's license is described as Copyright: © 2021 Bharadwaj et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.