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dc.contributor.authorPeterson, Nicholas D
dc.contributor.authorTse, Samantha Y
dc.contributor.authorHuang, Qiu Yu Judy
dc.contributor.authorWani, Khursheed A
dc.contributor.authorSchiffer, Celia A
dc.contributor.authorPukkila-Worley, Read
dc.date.accessioned2023-04-10T18:37:04Z
dc.date.available2023-04-10T18:37:04Z
dc.date.issued2023-02-15
dc.identifier.citationPeterson ND, Tse SY, Huang QJ, Wani KA, Schiffer CA, Pukkila-Worley R. Non-canonical pattern recognition of a pathogen-derived metabolite by a nuclear hormone receptor identifies virulent bacteria in C. elegans. Immunity. 2023 Feb 15:S1074-7613(23)00039-0. doi: 10.1016/j.immuni.2023.01.027. Epub ahead of print. PMID: 36804958.en_US
dc.identifier.eissn1097-4180
dc.identifier.doi10.1016/j.immuni.2023.01.027en_US
dc.identifier.pmid36804958
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51933
dc.description.abstractDistinguishing infectious pathogens from harmless microorganisms is essential for animal health. The mechanisms used to identify infectious microbes are not fully understood, particularly in metazoan hosts that eat bacteria as their food source. Here, we characterized a non-canonical pattern-recognition system in Caenorhabditis elegans (C. elegans) that assesses the relative threat of virulent Pseudomonas aeruginosa (P. Aeruginosa) to activate innate immunity. We discovered that the innate immune response in C. elegans was triggered by phenazine-1-carboxamide (PCN), a toxic metabolite produced by pathogenic strains of P. aeruginosa. We identified the nuclear hormone receptor NHR-86/HNF4 as the PCN sensor in C. elegans and validated that PCN bound to the ligand-binding domain of NHR-86/HNF4. Activation of NHR-86/HNF4 by PCN directly engaged a transcriptional program in intestinal epithelial cells that protected against P. aeruginosa. Thus, a bacterial metabolite is a pattern of pathogenesis surveilled by nematodes to identify a pathogen in its bacterial diet.en_US
dc.language.isoenen_US
dc.relation.ispartofImmunityen_US
dc.relation.urlhttps://doi.org/10.1016/j.immuni.2023.01.027en_US
dc.rightsCopyright 2023 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; Attribution-NonCommercial-NoDerivatives 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCaenorhabditis elegansen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjecthost-pathogen interactionsen_US
dc.subjectinnate immunityen_US
dc.subjectintestinal epithelial cellsen_US
dc.subjectnuclear hormone receptoren_US
dc.subjectpathogen sensingen_US
dc.subjectpattern recognition receptoren_US
dc.subjectpatterns of pathogenesisen_US
dc.subjectphenazinesen_US
dc.titleNon-canonical pattern recognition of a pathogen-derived metabolite by a nuclear hormone receptor identifies virulent bacteria in C. elegansen_US
dc.typeJournal Articleen_US
dc.source.journaltitleImmunity
dc.source.countryUnited States
dc.identifier.journalImmunity
refterms.dateFOA2023-04-10T18:37:04Z
dc.contributor.departmentBiochemistry and Molecular Biotechnologyen_US
dc.contributor.departmentMedicineen_US
dc.contributor.departmentMorningside Graduate School of Biomedical Sciencesen_US
dc.contributor.departmentProgram in Innate Immunityen_US
dc.contributor.departmentSchiffer Lab


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Copyright 2023 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as Copyright 2023 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; Attribution-NonCommercial-NoDerivatives 4.0 International