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dc.contributor.authorBala, Shashi
dc.contributor.authorGanz, Michal
dc.contributor.authorBabuta, Mrigya
dc.contributor.authorZhuang, Yuan
dc.contributor.authorCsak, Timea
dc.contributor.authorCalenda, Charles D
dc.contributor.authorSzabo, Gyongyi
dc.date.accessioned2023-04-25T13:30:23Z
dc.date.available2023-04-25T13:30:23Z
dc.date.issued2021-08-27
dc.identifier.citationBala S, Ganz M, Babuta M, Zhuang Y, Csak T, Calenda CD, Szabo G. Steatosis, inflammasome upregulation, and fibrosis are attenuated in miR-155 deficient mice in a high fat-cholesterol-sugar diet-induced model of NASH. Lab Invest. 2021 Dec;101(12):1540-1549. doi: 10.1038/s41374-021-00626-1. Epub 2021 Aug 27. PMID: 34453120; PMCID: PMC9272486.en_US
dc.identifier.eissn1530-0307
dc.identifier.doi10.1038/s41374-021-00626-1en_US
dc.identifier.pmid34453120
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51980
dc.description.abstractNonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease globally. miRNAs (miRs) regulate various cellular events that lead to NAFLD. In this study we tested the hypothesis that miR-155 is an important regulator of steatohepatitis and fibrosis pathways. Wild type (WT) or miR-155 deficient (KO) mice received a high fat-high cholesterol-high sugar-diet (HF-HC-HS) for 34 weeks and liver tissues were analyzed. In patients with nonalcoholic steatohepatitis and in the mouse model of HF-HC-HS diet we found increased miR-155 levels in the liver compared to normal livers. Upon HF-HC-HS diet feeding, miR-155 KO mice displayed less liver injury, decreased steatosis, and attenuation in fibrosis compared to WT mice. ALT, triglyceride levels, and genes involved in fatty acid metabolic pathway were increased in WT mice whereas miR-155 KO mice showed attenuation in these parameters. HF-HC-HS diet-induced significant increase in the expression of NLRP3 inflammasome components in the livers of WT mice compared to chow fed diet. Compared to WT mice, miR-155 KO showed attenuated induction in the NLRP3, ASC, and caspase1 inflammasome expression on HF-HC-HS diet. Fibrosis markers such as collagen content and deposition, αSMA, Zeb2, and vimentin were all increased in WT mice and miR-155 KO mice showed attenuated fibrosis marker expression. Overall, our findings highlight a role for miR-155 in HF-HC-HS diet-induced steatosis and liver fibrosis.en_US
dc.language.isoenen_US
dc.relation.ispartofLaboratory Investigationen_US
dc.relation.urlhttps://doi.org/10.1038/s41374-021-00626-1en_US
dc.rights© 2021. The Author(s), under exclusive licence to United States and Canadian Academy of Pathology.en_US
dc.titleSteatosis, inflammasome upregulation, and fibrosis are attenuated in miR-155 deficient mice in a high fat-cholesterol-sugar diet-induced model of NASHen_US
dc.typeJournal Articleen_US
dc.source.journaltitleLaboratory investigation; a journal of technical methods and pathology
dc.source.volume101
dc.source.issue12
dc.source.beginpage1540
dc.source.endpage1549
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalLaboratory investigation; a journal of technical methods and pathology
dc.contributor.departmentMedicineen_US


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