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dc.contributor.authorGainetdinov, Ildar
dc.contributor.authorColpan, Cansu
dc.contributor.authorCecchini, Katharine
dc.contributor.authorArif, Amena
dc.contributor.authorJouravleva, Karina
dc.contributor.authorAlbosta, Paul
dc.contributor.authorVega-Badillo, Joel
dc.contributor.authorLee, Yongjin
dc.contributor.authorÖzata, Deniz M
dc.contributor.authorZamore, Phillip D
dc.date.accessioned2023-04-25T13:32:43Z
dc.date.available2023-04-25T13:32:43Z
dc.date.issued2021-10-08
dc.identifier.citationGainetdinov I, Colpan C, Cecchini K, Arif A, Jouravleva K, Albosta P, Vega-Badillo J, Lee Y, Özata DM, Zamore PD. Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability. Mol Cell. 2021 Dec 2;81(23):4826-4842.e8. doi: 10.1016/j.molcel.2021.09.012. Epub 2021 Oct 8. PMID: 34626567; PMCID: PMC8642287.en_US
dc.identifier.eissn1097-4164
dc.identifier.doi10.1016/j.molcel.2021.09.012en_US
dc.identifier.pmid34626567
dc.identifier.urihttp://hdl.handle.net/20.500.14038/51981
dc.description.abstractIn animals, PIWI-interacting RNAs (piRNAs) silence transposons, fight viral infections, and regulate gene expression. piRNA biogenesis concludes with 3' terminal trimming and 2'-O-methylation. Both trimming and methylation influence piRNA stability. Our biochemical data show that multiple mechanisms destabilize unmethylated mouse piRNAs, depending on whether the piRNA 5' or 3' sequence is complementary to a trigger RNA. Unlike target-directed degradation of microRNAs, complementarity-dependent destabilization of piRNAs in mice and flies is blocked by 3' terminal 2'-O-methylation and does not require base pairing to both the piRNA seed and the 3' sequence. In flies, 2'-O-methylation also protects small interfering RNAs (siRNAs) from complementarity-dependent destruction. By contrast, pre-piRNA trimming protects mouse piRNAs from a degradation pathway unaffected by trigger complementarity. In testis lysate and in vivo, internal or 3' terminal uridine- or guanine-rich tracts accelerate pre-piRNA decay. Loss of both trimming and 2'-O-methylation causes the mouse piRNA pathway to collapse, demonstrating that these modifications collaborate to stabilize piRNAs.en_US
dc.language.isoenen_US
dc.relation.ispartofMolecular Cellen_US
dc.relation.urlhttps://doi.org/10.1016/j.molcel.2021.09.012en_US
dc.rightsCopyright © 2021 Elsevier Inc. All rights reserved.en_US
dc.subject2'-O-methylationen_US
dc.subjectPIWIen_US
dc.subjectRNA stabilityen_US
dc.subjectRNA turnoveren_US
dc.subjectpiRNAen_US
dc.subjectpiwi-interacting RNAen_US
dc.subjectsiRNAen_US
dc.subjectsmall RNAen_US
dc.subjectsmall interfering RNAen_US
dc.subjecttarget-directed microRNA degradationen_US
dc.titleTerminal modification, sequence, length, and PIWI-protein identity determine piRNA stabilityen_US
dc.typeJournal Articleen_US
dc.source.journaltitleMolecular cell
dc.source.volume81
dc.source.issue23
dc.source.beginpage4826
dc.source.endpage4842.e8
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalMolecular cell
dc.contributor.departmentRNA Therapeutics Instituteen_US


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