Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability
dc.contributor.author | Gainetdinov, Ildar | |
dc.contributor.author | Colpan, Cansu | |
dc.contributor.author | Cecchini, Katharine | |
dc.contributor.author | Arif, Amena | |
dc.contributor.author | Jouravleva, Karina | |
dc.contributor.author | Albosta, Paul | |
dc.contributor.author | Vega-Badillo, Joel | |
dc.contributor.author | Lee, Yongjin | |
dc.contributor.author | Özata, Deniz M | |
dc.contributor.author | Zamore, Phillip D | |
dc.date.accessioned | 2023-04-25T13:32:43Z | |
dc.date.available | 2023-04-25T13:32:43Z | |
dc.date.issued | 2021-10-08 | |
dc.identifier.citation | Gainetdinov I, Colpan C, Cecchini K, Arif A, Jouravleva K, Albosta P, Vega-Badillo J, Lee Y, Özata DM, Zamore PD. Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability. Mol Cell. 2021 Dec 2;81(23):4826-4842.e8. doi: 10.1016/j.molcel.2021.09.012. Epub 2021 Oct 8. PMID: 34626567; PMCID: PMC8642287. | en_US |
dc.identifier.eissn | 1097-4164 | |
dc.identifier.doi | 10.1016/j.molcel.2021.09.012 | en_US |
dc.identifier.pmid | 34626567 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/51981 | |
dc.description.abstract | In animals, PIWI-interacting RNAs (piRNAs) silence transposons, fight viral infections, and regulate gene expression. piRNA biogenesis concludes with 3' terminal trimming and 2'-O-methylation. Both trimming and methylation influence piRNA stability. Our biochemical data show that multiple mechanisms destabilize unmethylated mouse piRNAs, depending on whether the piRNA 5' or 3' sequence is complementary to a trigger RNA. Unlike target-directed degradation of microRNAs, complementarity-dependent destabilization of piRNAs in mice and flies is blocked by 3' terminal 2'-O-methylation and does not require base pairing to both the piRNA seed and the 3' sequence. In flies, 2'-O-methylation also protects small interfering RNAs (siRNAs) from complementarity-dependent destruction. By contrast, pre-piRNA trimming protects mouse piRNAs from a degradation pathway unaffected by trigger complementarity. In testis lysate and in vivo, internal or 3' terminal uridine- or guanine-rich tracts accelerate pre-piRNA decay. Loss of both trimming and 2'-O-methylation causes the mouse piRNA pathway to collapse, demonstrating that these modifications collaborate to stabilize piRNAs. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Molecular Cell | en_US |
dc.relation.url | https://doi.org/10.1016/j.molcel.2021.09.012 | en_US |
dc.rights | Copyright © 2021 Elsevier Inc. All rights reserved. | en_US |
dc.subject | 2'-O-methylation | en_US |
dc.subject | PIWI | en_US |
dc.subject | RNA stability | en_US |
dc.subject | RNA turnover | en_US |
dc.subject | piRNA | en_US |
dc.subject | piwi-interacting RNA | en_US |
dc.subject | siRNA | en_US |
dc.subject | small RNA | en_US |
dc.subject | small interfering RNA | en_US |
dc.subject | target-directed microRNA degradation | en_US |
dc.title | Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability | en_US |
dc.type | Journal Article | en_US |
dc.source.journaltitle | Molecular cell | |
dc.source.volume | 81 | |
dc.source.issue | 23 | |
dc.source.beginpage | 4826 | |
dc.source.endpage | 4842.e8 | |
dc.source.country | United States | |
dc.source.country | United States | |
dc.source.country | United States | |
dc.source.country | United States | |
dc.source.country | United States | |
dc.source.country | United States | |
dc.identifier.journal | Molecular cell | |
dc.contributor.department | RNA Therapeutics Institute | en_US |