Show simple item record

dc.contributor.authorDutta, Debabrata
dc.contributor.authorNguyen, Vu
dc.contributor.authorCampbell, Kenneth S
dc.contributor.authorPadrón, Raúl
dc.contributor.authorCraig, Roger
dc.date.accessioned2023-05-02T18:45:31Z
dc.date.available2023-05-02T18:45:31Z
dc.date.issued2023-04-12
dc.identifier.citationDutta D, Nguyen V, Campbell KS, Padrón R, Craig R. Cryo-EM structure of the human cardiac myosin filament. bioRxiv [Preprint]. 2023 Apr 12:2023.04.11.536274. doi: 10.1101/2023.04.11.536274. PMID: 37090534; PMCID: PMC10120621.en_US
dc.identifier.doi10.1101/2023.04.11.536274en_US
dc.identifier.pmid37090534
dc.identifier.urihttp://hdl.handle.net/20.500.14038/52021
dc.descriptionThis article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.en_US
dc.description.abstractPumping of the heart is powered by filaments of the motor protein myosin, which pull on actin filaments to generate cardiac contraction. In addition to myosin, the filaments contain cardiac myosin-binding protein C (cMyBP-C), which modulates contractility in response to physiological stimuli, and titin, which functions as a scaffold for filament assembly 1 . Myosin, cMyBP-C and titin are all subject to mutation, which can lead to heart failure. Despite the central importance of cardiac myosin filaments to life, their molecular structure has remained a mystery for 60 years 2 . Here, we have solved the structure of the main (cMyBP-C-containing) region of the human cardiac filament to 6 Å resolution by cryo-EM. The reconstruction reveals the architecture of titin and cMyBP-C for the first time, and shows how myosin's motor domains (heads) form 3 different types of motif (providing functional flexibility), which interact with each other and with specific domains of titin and cMyBP-C to dictate filament architecture and regulate function. A novel packing of myosin tails in the filament backbone is also resolved. The structure suggests how cMyBP-C helps generate the cardiac super-relaxed state 3 , how titin and cMyBP-C may contribute to length-dependent activation 4 , and how mutations in myosin and cMyBP-C might disrupt interactions, causing disease 5, 6 . A similar structure is likely in vertebrate skeletal myosin filaments. The reconstruction resolves past uncertainties, and integrates previous data on cardiac muscle structure and function. It provides a new paradigm for interpreting structural, physiological and clinical observations, and for the design of potential therapeutic drugs.en_US
dc.language.isoenen_US
dc.relationNow published in Nature, https://doi.org/10.1038/s41586-023-06691-4
dc.relation.ispartofbioRxiven_US
dc.relation.urlhttps://doi.org/10.1101/2023.04.11.536274en_US
dc.rightsThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.en_US
dc.subjectBiophysicsen_US
dc.subjectmyosinen_US
dc.titleCryo-EM structure of the human cardiac myosin filament [preprint]en_US
dc.typePreprinten_US
dc.source.journaltitlebioRxiv : the preprint server for biology
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalbioRxiv : the preprint server for biology
dc.contributor.departmentRadiologyen_US
dc.contributor.departmentPadrón-Craig Lab


This item appears in the following Collection(s)

Show simple item record