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dc.contributor.authorZhong, Jianjun
dc.contributor.authorGunner, Georgia
dc.contributor.authorHenninger, Nils
dc.contributor.authorSchafer, Dorothy P
dc.contributor.authorBosco, Daryl A
dc.date.accessioned2023-07-14T18:54:12Z
dc.date.available2023-07-14T18:54:12Z
dc.date.issued2023-04-21
dc.identifier.citationZhong J, Gunner G, Henninger N, Schafer DP, Bosco DA. Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope. J Vis Exp. 2023 Apr 21;(194). doi: 10.3791/64701. PMID: 37154548.en_US
dc.identifier.eissn1940-087X
dc.identifier.doi10.3791/64701en_US
dc.identifier.pmid37154548
dc.identifier.urihttp://hdl.handle.net/20.500.14038/52293
dc.description.abstractThe goal of this protocol is to demonstrate how to longitudinally visualize the expression and localization of a protein of interest within specific cell types of an animal's brain, upon exposure to exogenous stimuli. Here, the administration of a closed-skull traumatic brain injury (TBI) and simultaneous implantation of a cranial window for subsequent longitudinal intravital imaging in mice is shown. Mice are intracranially injected with an adeno-associated virus (AAV) expressing enhanced green fluorescent protein (EGFP) under a neuronal specific promoter. After 2 to 4 weeks, the mice are subjected to a repetitive TBI using a weight drop device over the AAV injection location. Within the same surgical session, the mice are implanted with a metal headpost and then a glass cranial window over the TBI impacting site. The expression and cellular localization of EGFP is examined using a two-photon microscope in the same brain region exposed to trauma over the course of months.en_US
dc.language.isoenen_US
dc.relation.ispartofJoVEen_US
dc.relation.urlhttps://doi.org/10.3791/64701en_US
dc.titleIntravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscopeen_US
dc.typeJournal Articleen_US
dc.source.journaltitleJournal of visualized experiments : JoVE
dc.source.issue194
dc.source.countryUnited States
dc.identifier.journalJournal of visualized experiments : JoVE
dc.contributor.departmentMorningside Graduate School of Biomedical Sciencesen_US
dc.contributor.departmentNeurobiologyen_US
dc.contributor.departmentNeurologyen_US
dc.contributor.departmentSchafer Lab
dc.contributor.studentGeorgia Gunner
dc.description.thesisprogramNeuroscience


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