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dc.contributor.authorGong, Jiaxin
dc.contributor.authorNirala, Niraj K
dc.contributor.authorChen, Jiazhang
dc.contributor.authorWang, Fei
dc.contributor.authorGu, Pengyu
dc.contributor.authorWen, Qi
dc.contributor.authorIp, Y Tony
dc.contributor.authorXiang, Yang
dc.date.accessioned2023-07-14T19:08:43Z
dc.date.available2023-07-14T19:08:43Z
dc.date.issued2023-05-24
dc.identifier.citationGong J, Nirala NK, Chen J, Wang F, Gu P, Wen Q, Ip YT, Xiang Y. TrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation in Drosophila. Sci Adv. 2023 May 24;9(21):eadc9660. doi: 10.1126/sciadv.adc9660. Epub 2023 May 24. PMID: 37224252; PMCID: PMC10208578.en_US
dc.identifier.eissn2375-2548
dc.identifier.doi10.1126/sciadv.adc9660en_US
dc.identifier.pmid37224252
dc.identifier.urihttp://hdl.handle.net/20.500.14038/52297
dc.description.abstractAdult stem cells are essential for tissue maintenance and repair. Although genetic pathways for controlling adult stem cells are extensively investigated in various tissues, much less is known about how mechanosensing could regulate adult stem cells and tissue growth. Here, we demonstrate that shear stress sensing regulates intestine stem cell proliferation and epithelial cell number in adult Drosophila. Ca2+ imaging in ex vivo midguts shows that shear stress, but not other mechanical forces, specifically activates enteroendocrine cells among all epithelial cell types. This activation is mediated by transient receptor potential A1 (TrpA1), a Ca2+-permeable channel expressed in enteroendocrine cells. Furthermore, specific disruption of shear stress, but not chemical, sensitivity of TrpA1 markedly reduces proliferation of intestinal stem cells and midgut cell number. Therefore, we propose that shear stress may act as a natural mechanical stimulation to activate TrpA1 in enteroendocrine cells, which, in turn, regulates intestine stem cell behavior.en_US
dc.language.isoenen_US
dc.relation.ispartofScience Advancesen_US
dc.relation.urlhttps://doi.org/10.1126/sciadv.adc9660en_US
dc.rightsCopyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).en_US
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.titleTrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation in Drosophilaen_US
dc.typeJournal Articleen_US
dc.source.journaltitleScience advances
dc.source.volume9
dc.source.issue21
dc.source.beginpageeadc9660
dc.source.endpage
dc.source.countryUnited States
dc.identifier.journalScience advances
refterms.dateFOA2023-07-14T19:08:45Z
dc.contributor.departmentNeurobiologyen_US
dc.contributor.departmentProgram in Molecular Medicineen_US
dc.contributor.departmentYang Xiang Lab


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Copyright © 2023 The
Authors, some
rights reserved;
exclusive licensee
American Association
for the Advancement
of Science. No claim to
original U.S. Government
Works. Distributed
under a Creative
Commons Attribution
NonCommercial
License 4.0 (CC BY-NC).
Except where otherwise noted, this item's license is described as Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).