Visualizing everything, everywhere, all at once: Cryo-EM and the new field of structureomics
AuthorsLucas, Bronwyn A
UMass Chan AffiliationsRNA Therapeutics Institute
Document TypeJournal Article
MetadataShow full item record
AbstractTwenty years ago, the release of the first draft of the human genome sequence instigated a paradigm shift in genomics and molecular biology. Arguably, structural biology is entering an analogous era, with availability of an experimentally determined or predicted molecular model for almost every protein-coding gene from many genomes-producing a reference "structureome". Structural predictions require experimental validation and not all proteins conform to a single structure, making any reference structureome necessarily incomplete. Despite these limitations, a reference structureome can be used to characterize cell state in more detail than by quantifying sequence or expression levels alone. Cryogenic electron microscopy (cryo-EM) is a method that can generate atomic resolution views of molecules and cells frozen in place. In this perspective I consider how emerging cryo-EM methods are contributing to the new field of structureomics.
SourceLucas BA. Visualizing everything, everywhere, all at once: Cryo-EM and the new field of structureomics. Curr Opin Struct Biol. 2023 Jun 4;81:102620. doi: 10.1016/j.sbi.2023.102620. Epub ahead of print. PMID: 37279614.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/52333
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Except where otherwise noted, this item's license is described as © 2023 The Author. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).