AuthorsFaust, Travis E
Feinberg, Philip A
Boyle, Margaret A
Schafer, Dorothy P
Student AuthorsPhilip Feinberg
UMass Chan AffiliationsBrudnick Neuropsychiatric Research Institute
Morningside Graduate School of Biomedical Sciences
Document TypeJournal Article
MetadataShow full item record
AbstractCre/loxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. Microglial biology has particularly benefited because microglia historically have been difficult to transduce with virus or electroporation methods for gene delivery. Here, we investigate five of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency, cell-type specificity, and spontaneous recombination, depending on the Cre line and inter-loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency, particularly in microglia. There is increasing evidence that microglia are key regulators of neural circuits and major drivers of a broad range of neurological diseases. Reliable manipulation of their function in vivo is of utmost importance. Identifying caveats and benefits of all tools and implementing the most rigorous protocols are crucial to the growth of the field and the development of microglia-based therapeutics.
SourceFaust TE, Feinberg PA, O'Connor C, Kawaguchi R, Chan A, Strasburger H, Frosch M, Boyle MA, Masuda T, Amann L, Knobeloch KP, Prinz M, Schaefer A, Schafer DP. A comparative analysis of microglial inducible Cre lines. Cell Rep. 2023 Aug 26;42(9):113031. doi: 10.1016/j.celrep.2023.113031. Epub ahead of print. PMID: 37635351.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/52572
Related ResourcesThis article is based on a previously available preprint in bioRxiv, https://doi.org/10.1101/2023.01.09.523268
RightsCopyright © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; Attribution-NonCommercial-NoDerivatives 4.0 International
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Except where otherwise noted, this item's license is described as Copyright © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).