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dc.contributor.authorLi, Huilin
dc.contributor.authorO'Donnell, Mike
dc.contributor.authorKelch, Brian A
dc.date.accessioned2024-01-16T17:44:11Z
dc.date.available2024-01-16T17:44:11Z
dc.date.issued2022-09-18
dc.identifier.citationLi H, O'Donnell M, Kelch B. Unexpected new insights into DNA clamp loaders: Eukaryotic clamp loaders contain a second DNA site for recessed 5' ends that facilitates repair and signals DNA damage: Eukaryotic clamp loaders contain a second DNA site for recessed 5' ends that facilitates repair and signals DNA damage. Bioessays. 2022 Nov;44(11):e2200154. doi: 10.1002/bies.202200154. Epub 2022 Sep 18. PMID: 36116108; PMCID: PMC9927785.en_US
dc.identifier.eissn1521-1878
dc.identifier.doi10.1002/bies.202200154en_US
dc.identifier.pmid36116108
dc.identifier.urihttp://hdl.handle.net/20.500.14038/52969
dc.description.abstractClamp loaders are pentameric AAA+ assemblies that use ATP to open and close circular DNA sliding clamps around DNA. Clamp loaders show homology in all organisms, from bacteria to human. The eukaryotic PCNA clamp is loaded onto 3' primed DNA by the replication factor C (RFC) hetero-pentameric clamp loader. Eukaryotes also have three alternative RFC-like clamp loaders (RLCs) in which the Rfc1 subunit is substituted by another protein. One of these is the yeast Rad24-RFC (Rad17-RFC in human) that loads a 9-1-1 heterotrimer clamp onto a recessed 5' end of DNA. Recent structural studies of Rad24-RFC have discovered an unexpected 5' DNA binding site on the outside of the clamp loader and reveal how a 5' end can be utilized for loading the 9-1-1 clamp onto DNA. In light of these results, new studies reveal that RFC also contains a 5' DNA binding site, which functions in gap repair. These studies also reveal many new features of clamp loaders. As reviewed herein, these recent studies together have transformed our view of the clamp loader mechanism.en_US
dc.language.isoenen_US
dc.relation.ispartofBioessaysen_US
dc.relation.urlhttps://doi.org/10.1002/bies.202200154en_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2022 The Authors. BioEssays published by Wiley Periodicals LLCen_US
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subject9-1-1 clampen_US
dc.subjectAAA+ oligomersen_US
dc.subjectDNA repairen_US
dc.subjectDNA replicationen_US
dc.subjectPCNA clampen_US
dc.subjectclamp loaderen_US
dc.subjectsliding clampen_US
dc.titleUnexpected new insights into DNA clamp loaders: Eukaryotic clamp loaders contain a second DNA site for recessed 5' ends that facilitates repair and signals DNA damage: Eukaryotic clamp loaders contain a second DNA site for recessed 5' ends that facilitates repair and signals DNA damageen_US
dc.typeJournal Articleen_US
dc.source.journaltitleBioEssays : news and reviews in molecular, cellular and developmental biology
dc.source.volume44
dc.source.issue11
dc.source.beginpagee2200154
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalBioEssays : news and reviews in molecular, cellular and developmental biology
refterms.dateFOA2024-01-16T17:44:13Z
dc.contributor.departmentBiochemistry and Molecular Biotechnologyen_US


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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any
medium, provided the original work is properly cited and is not used for commercial purposes.
© 2022 The Authors. BioEssays published by Wiley Periodicals LLC
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2022 The Authors. BioEssays published by Wiley Periodicals LLC