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dc.contributor.authorFaust, Travis E
dc.contributor.authorLee, Yi-Han
dc.contributor.authorO'Connor, Ciara
dc.contributor.authorBoyle, Margaret A
dc.contributor.authorGunner, Georgia
dc.contributor.authorBadimon, Ana
dc.contributor.authorAyata, Pinar
dc.contributor.authorSchaefer, Anne
dc.contributor.authorSchafer, Dorothy P
dc.date.accessioned2024-03-29T14:33:57Z
dc.date.available2024-03-29T14:33:57Z
dc.date.issued2024-02-09
dc.identifier.citationFaust TE, Lee YH, O'Connor CD, Boyle MA, Gunner G, Badimon A, Ayata P, Schaefer A, Schafer DP. Microglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling. bioRxiv [Preprint]. 2024 Feb 9:2024.02.08.579178. doi: 10.1101/2024.02.08.579178. PMID: 38370645; PMCID: PMC10871360.en_US
dc.identifier.doi10.1101/2024.02.08.579178en_US
dc.identifier.pmid38370645
dc.identifier.urihttp://hdl.handle.net/20.500.14038/53250
dc.descriptionThis article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.en_US
dc.description.abstractAstrocytes and microglia are emerging key regulators of activity-dependent synapse remodeling that engulf and remove synapses in response to changes in neural activity. Yet, the degree to which these cells communicate to coordinate this process remains an open question. Here, we use whisker removal in postnatal mice to induce activity-dependent synapse removal in the barrel cortex. We show that astrocytes do not engulf synapses in this paradigm. Instead, astrocytes reduce their contact with synapses prior to microglia-mediated synapse engulfment. We further show that reduced astrocyte-contact with synapses is dependent on microglial CX3CL1-CX3CR1 signaling and release of Wnts from microglia following whisker removal. These results demonstrate an activity-dependent mechanism by which microglia instruct astrocyte-synapse interactions, which then provides a permissive environment for microglia to remove synapses. We further show that this mechanism is critical to remodel synapses in a changing sensory environment and this signaling is upregulated in several disease contexts.en_US
dc.language.isoenen_US
dc.relation.ispartofbioRxiven_US
dc.relation.urlhttps://doi.org/10.1101/2024.02.08.579178en_US
dc.rightsThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMicrogliaen_US
dc.subjectWnten_US
dc.subjectastrocyteen_US
dc.subjectsynapse remodelingen_US
dc.titleMicroglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling [preprint]en_US
dc.typePreprinten_US
dc.source.journaltitlebioRxiv : the preprint server for biology
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.identifier.journalbioRxiv : the preprint server for biology
refterms.dateFOA2024-03-29T14:33:59Z
dc.contributor.departmentBrudnick Neuropsychiatric Research Instituteen_US
dc.contributor.departmentMorningside Graduate School of Biomedical Sciencesen_US
dc.contributor.departmentNeurobiologyen_US
dc.contributor.departmentSchafer Lab
dc.contributor.studentYi-Han Lee
dc.description.thesisprogramNeuroscience


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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.