Magnelli, PaulaCipollo, John F.Ratner, Daniel M.Cui, JikeKelleher, Daniel J.Gilmore, ReidCostello, Catherine E.Robbins, Phillips W.Samuelson, John2022-08-232022-08-232008-06-272014-10-22J Biol Chem. 2008 Jun 27;283(26):18355-64. doi: 10.1074/jbc.M800725200. Epub 2008 Apr 16.<a href="http://dx.doi.org/10.1074/jbc.M800725200">Link to article on publisher's site</a>0021-9258 (Linking)10.1074/jbc.M80072520018417475https://hdl.handle.net/20.500.14038/26042N-Glycans of Entamoeba histolytica, the protist that causes amebic dysentery and liver abscess, are of great interest for multiple reasons. E. histolytica makes an unusual truncated N-glycan precursor (Man(5)GlcNAc(2)), has few nucleotide sugar transporters, and has a surface that is capped by the lectin concanavalin A. Here, biochemical and mass spectrometric methods were used to examine N-glycan biosynthesis and the final N-glycans of E. histolytica with the following conclusions. Unprocessed Man(5)GlcNAc(2), which is the most abundant E. histolytica N-glycan, is aggregated into caps on the surface of E. histolytica by the N-glycan-specific, anti-retroviral lectin cyanovirin-N. Glc(1)Man(5)GlcNAc(2), which is made by a UDP-Glc: glycoprotein glucosyltransferase that is part of a conserved N-glycan-dependent endoplasmic reticulum quality control system for protein folding, is also present in mature N-glycans. A swainsonine-sensitive alpha-mannosidase trims some N-glycans to biantennary Man(3)GlcNAc(2). Complex N-glycans of E. histolytica are made by the addition of alpha1,2-linked Gal to both arms of small oligomannose glycans, and Gal residues are capped by one or more Glc. In summary, E. histolytica N-glycans include unprocessed Man(5)GlcNAc(2), which is a target for cyanovirin-N, as well as unique, complex N-glycans containing Gal and Glc.en-USAnimalsAsparagineConcanavalin AEndoplasmic ReticulumEntamoeba histolytica*Gene Expression RegulationGlycosylationLectinsMass SpectrometryModels, ChemicalMolecular ConformationOligosaccharidesPolysaccharidesProtein Foldingalpha-MannosidaseBiochemistryBiochemistry, Biophysics, and Structural BiologyMolecular BiologyJournal Articlehttps://escholarship.umassmed.edu/bmp_pp/1816269454bmp_pp/181