Zou, HuiUgur, MehmetDrummond, Robert M.Singer, Joshua J.2022-08-232022-08-232001-07-042009-03-26<p>J Physiol. 2001 Jul 1;534(Pt 1):59-70.</p>0022-3751 (Print)10.1111/j.1469-7793.2001.00059.x11432992https://hdl.handle.net/20.500.14038/386261. Extracellular application of ATP generates two whole-cell currents in toad gastric smooth muscle cells: an immediate inward non-selective cation current (due to the activation of a P2X or P2Z-like receptor) and a slowly developing outward K(+) current. The inward non-selective cation current depends on the continuous presence of ATP while the outward K(+) current can last for minutes after ATP application ceases. 2. In cell-attached patches, application of ATP to the extra-patch membrane can activate K(+) channels in the patch indicating that a diffusible cellular messenger may be involved. The characteristics of these K(+) channels are similar to those of a previously described fatty acid-activated K(+) channel that is also a stretch-activated channel. 3. This whole-cell K(+) current can be induced by ATP in the absence of extracellular Ca(2+) (with EGTA present to chelate trace amounts). However, the current generated in the presence of extracellular Ca(2+) is considerably larger. 4. The pharmacological profiles for the activation of the non-selective cation current and the K(+) current are similar, suggesting that the same P2Z-like receptor could be mediating both responses. This type of plasma membrane receptor/channel-channel coupling by a process that does not appear to involve Ca(2+) flow through the receptor/channel or a subsequent membrane potential change may be representative of a new class of signalling mechanisms.en-USAdenosine TriphosphateAnimalsBufo marinusCalciumCell MembraneElectric ConductivityElectrophysiologyExtracellular SpaceFatty AcidsGTP-Binding ProteinsIntracellular FluidMuscle, SmoothOsmolar ConcentrationPatch-Clamp TechniquesPotassium ChannelsReceptors, Purinergic P2StomachLife SciencesMedicine and Health SciencesJournal Articlehttps://escholarship.umassmed.edu/oapubs/1488798463oapubs/1488