Reece-Hoyes, John S.Walhout, Albertha J M2022-08-232022-08-232018-07-022018-09-19<p title="Cold Spring Harbor protocols.">Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.top094953. doi: 10.1101/pdb.top094953. <a href="https://doi.org/10.1101/pdb.top094953">Link to article on publisher's site</a></p>1559-6095 (Linking)10.1101/pdb.top09495329967278https://hdl.handle.net/20.500.14038/49868In the first section of this introduction, we provide background information for yeast two-hybrid (Y2H) assays that provide a genetic method for the identification and analysis of binary protein-protein interactions and that are complementary to biochemical methods such as immunoprecipitation. In the second section, we discuss yeast one-hybrid (Y1H) assays that provide a "gene-centered" (DNA-to-protein) genetic method to identify and study protein-DNA interactions between cis-regulatory elements and transcription factors (TFs). This method is complementary to "TF-centered" (protein-to-DNA) biochemical methods such as chromatin immunoprecipitation.en-USBiochemistry, Biophysics, and Structural BiologyGenetics and GenomicsLaboratory and Basic Science ResearchSystems BiologyJournal Articlehttps://escholarship.umassmed.edu/sysbio_pubs/14012900771sysbio_pubs/140