Sluder, GreenfieldNordberg, Joshua J.2022-08-232022-08-232013-08-072014-06-10Sluder G, Nordberg JJ. Microscope basics. Methods Cell Biol. 2013;114:1-10. doi: 10.1016/B978-0-12-407761-4.00001-4. <a href="http://dx.doi.org/10.1016/B978-0-12-407761-4.00001-4">Link to article on publisher's site</a>0091-679X (Linking)10.1016/B978-0-12-407761-4.00001-423931500https://hdl.handle.net/20.500.14038/49028This chapter provides information on how microscopes work and discusses some of the microscope issues to be considered in using a video camera on the microscope. There are two types of microscopes in use today for research in cell biology-the older finite tube-length (typically 160mm mechanical tube length) microscopes and the infinity optics microscopes that are now produced. The objective lens forms a magnified, real image of the specimen at a specific distance from the objective known as the intermediate image plane. All objectives are designed to be used with the specimen at a defined distance from the front lens element of the objective (the working distance) so that the image formed is located at a specific location in the microscope. Infinity optics microscopes differ from the finite tube-length microscopes in that the objectives are designed to project the image of the specimen to infinity and do not, on their own, form a real image of the specimen. Three types of objectives are in common use today-plan achromats, plan apochromats, and plan fluorite lenses. The concept of mounting video cameras on the microscope is also presented in the chapter.en-USMicroscopy, FluorescenceMicroscopy, VideoOptical PhenomenaVideo RecordingCell BiologyInvestigative TechniquesLaboratory and Basic Science ResearchOpticsJournal Articlehttps://escholarship.umassmed.edu/sluder/155676440sluder/15