Alugupalli, Kishore R.Gerstein, Rachel M.Chen, JianzhuSzomolanyi-Tsuda, EvaWoodland, Robert T.Leong, John M.2022-08-232022-08-232003-03-212009-03-10<p>J Immunol. 2003 Apr 1;170(7):3819-27.</p>0022-1767 (Print)10.4049/jimmunol.170.7.381912646649https://hdl.handle.net/20.500.14038/38239The rate of pathogen clearance is a critical determinant of morbidity and mortality. We sought to characterize the immune response responsible for the remarkably rapid clearance of individual episodes of bacteremia caused by the relapsing fever bacterium, Borrelia hermsii. SCID or Rag(-/-) mice were incapable of resolving B. hermsii infection, indicating a critical role for T and/or B cells. TCR(-/-) mice, which lack T cells, and IL-7(-/-) mice, which are deficient in both T cells and follicular B cells, but not in B1 cells and splenic marginal zone (MZ) B cells, efficiently cleared B. hermsii. These findings suggested that B1 cells and/or MZ B cells, two B cell subsets that are known to participate in rapid, T-independent responses, might be involved. The efficient resolution of the episodes of moderate level bacteremia by splenectomized mice suggested that MZ B cells do not play the primary role in clearance of this bacterium. In contrast, xid mice, which are deficient in B1 cells, suffered more severe episodes of bacteremia than wild-type mice. The hypothesis that B1 cells are critical for clearance of B. hermsii was further supported by a selective expansion of the B1b (i.e., IgM(high), IgD(-/low), Mac1(+) CD23(-), and CD5(-)) cell subset in infected xid mice, which coincided with the eventual resolution of infection. Finally, mice selectively incapable of secreting IgM, the dominant isotype produced by B1 cells, were completely unable to clear B. hermsii. Together these results support the model that B1b cells generate the T-independent IgM required for the control and resolution of relapsing fever borreliosis.en-USAnimalsB-Lymphocyte SubsetsBacteremiaBlood Bactericidal ActivityBorreliaCell DifferentiationImmunity, NaturalImmunoglobulin MLymphocyte ActivationLymphopeniaMiceMice, Inbred C57BLMice, Inbred CBAMice, KnockoutMice, Mutant StrainsMice, SCIDRelapsing FeverSpleenT-LymphocytesLife SciencesMedicine and Health SciencesJournal Articlehttps://escholarship.umassmed.edu/oapubs/1114770092oapubs/1114