Moriarty, Charlotte M. HarwoodPursell, Bryan M.Mercurio, Arthur M.2022-08-232022-08-232010-07-022010-11-07Moriarty CH, Pursell B, Mercurio AM. miR-10b targets Tiam1: implications for Rac activation and carcinoma migration. J Biol Chem. 2010 Jul 2;285(27):20541-6. Epub 2010 May 5. <a href="http://dx.doi.org/10.1074/jbc.M110.121012">Link to article on publisher's website</a>0021-9258 (Linking)10.1074/jbc.M110.12101220444703https://hdl.handle.net/20.500.14038/26279<p>Co-author Charlotte Moriarty is a GSBS student in the Cancer Biology and MD/PhD programs in the Graduate School of Biomedical Sciences at UMass Medical School.</p>Understanding the mechanisms by which specific microRNAs regulate cell migration and invasion is a timely and significant problem in cancer cell biology. miR-10b is of interest in this regard because its expression is altered in breast and other cancers. Our analysis of potential miR-10b targets identified Tiam1 (T lymphoma invasion and metastasis 1), a guanidine exchange factor for Rac. We demonstrate, using an miR-10b synthetic precursor, expression vector, and antisense oligonucleotide, that miR-10b represses Tiam1 expression in breast carcinoma cells and that it interacts with the 3'-UTR of Tiam1. Consistent with the involvement of Tiam1 in cell motility, we observed that miR-10b suppresses the ability of breast carcinoma cells to migrate and invade. Importantly, we demonstrate that miR-10b also inhibits Tiam1-mediated Rac activation. These data provide a mechanism for the regulation of Tiam1-mediated Rac activation in breast cancer cells and need to be considered in the context of other reported functions for miR-10b.en-US3' Untranslated RegionsBreast NeoplasmsCell Line, TumorCell MovementFemaleGenes, ReporterGuanine Nucleotide Exchange FactorsinhibitorsHumansLuciferasesMicroRNAsNeoplasm InvasivenessNeoplasmsPolymerase Chain ReactionRNA, Neoplasmrac GTP-Binding ProteinsCancer BiologyNeoplasmsJournal Articlehttps://escholarship.umassmed.edu/cancerbiology_pp/1931633419cancerbiology_pp/193