Reece-Hoyes, John S.Walhout, Albertha J M2022-08-232022-08-232018-07-022018-09-19<p>Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.prot094995. doi: 10.1101/pdb.prot094995.<a href="https://doi.org/10.1101/pdb.prot094995">Link to article on publisher's site</a></p>1559-6095 (Linking)10.1101/pdb.prot09499529967274https://hdl.handle.net/20.500.14038/49869High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.en-USGenetics and GenomicsLaboratory and Basic Science ResearchSystems BiologyJournal Articlehttps://escholarship.umassmed.edu/sysbio_pubs/14112900773sysbio_pubs/141