Flynn, Mackenzie JBenanti, Jennifer A2023-11-072023-11-072022-06-02Flynn MJ, Benanti JA. Cip1 tunes cell cycle arrest duration upon calcineurin activation. Proc Natl Acad Sci U S A. 2022 Jun 7;119(23):e2202469119. doi: 10.1073/pnas.2202469119. Epub 2022 Jun 2. PMID: 35653562; PMCID: PMC9191682.1091-649010.1073/pnas.220246911935653562https://hdl.handle.net/20.500.14038/52716Cells exposed to environmental stress arrest the cell cycle until they have adapted to their new environment. Cells adjust the length of the arrest for each unique stressor, but how they do this is not known. Here, we investigate the role of the stress-activated phosphatase calcineurin (CN) in controlling cell cycle arrest in Saccharomyces cerevisiae. We find that CN controls arrest duration through activation of the G1 cyclin–dependent kinase inhibitor Cip1. Our results demonstrate that multiple stressors trigger a G1/S arrest through Hog1-dependent down-regulation of G1 cyclin transcription. When a stressor also activates CN, this arrest is lengthened as CN prolongs Hog1-dependent phosphorylation of Cip1. Cip1 plays no role in response to stressors that activate Hog1 but not CN. These findings illustrate how stress response pathways cooperate to tailor the stress response and suggest that Cip1 functions to prolong cell cycle arrest when a cell requires additional time for adaptation.enCopyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Cip1calcineurincell cyclestress responseJournal ArticleProceedings of the National Academy of Sciences of the United States of America