Lack of alternative coreceptor use by pediatric HIV-1 R5 isolates for infection of primary cord or adult peripheral blood mononuclear cells

Sullivan, W. MatthewDorr, P.Perros, M.Hudson, Richard W. Jr.Leif, Jean H.Luzuriaga, KatherineClapham, Paul R.2022-08-232022-08-232008-01-012012-05-01Arch Virol. 2008;153(2):363-6. Epub 2007 Dec 13. <a href="http://dx.doi.org/10.1007/s00705-007-1099-6">Link to article on publisher's site</a>0304-8608 (Linking)10.1007/s00705-007-1099-618074097https://hdl.handle.net/20.500.14038/43478HIV-1 infection of neonates results in an extended acute period of virus replication, frequent neurological problems and reduced survival compared to adults. In adults, R5 viruses mainly infect CCR5(+) CD4(+) memory T-cells. In neonates, CCR5(+) memory T-cells form a substantially smaller fraction of total lymphocytes. We therefore tested whether alternative coreceptors confer infection of lymphocytes by pediatric isolates. Pediatric HIV-1 R5 isolates failed to replicate in Delta32/Delta32 CCR5 PBMCs or in cord PBMCs treated with a CCR5 inhibitor. These results do not indicate a role for alternative coreceptors and provide support for CCR5 inhibitors in the therapy of HIV-1(+) neonates.en-USHIV InfectionsHIV Reverse TranscriptaseHIV-1HumansInfantInfant, NewbornLeukocytes, MononuclearReceptors, CCR5Receptors, Virus*Virus InternalizationVirus ReplicationImmunology and Infectious DiseasePediatricsLack of alternative coreceptor use by pediatric HIV-1 R5 isolates for infection of primary cord or adult peripheral blood mononuclear cellsJournal Articlehttps://escholarship.umassmed.edu/peds_immunology/592814390peds_immunology/59