Owa, MikitoFuruta, AkaneUsukura, JiroArisaka, FumioKing, Stephen M.Witman, George B.Kamiya, RitsuWakabayashi, Ken-ichi2022-08-232022-08-232014-07-012015-04-01<p>Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9461-6. doi: 10.1073/pnas.1403101111. Epub 2014 Jun 16. <a href="http://dx.doi.org/10.1073/pnas.1403101111">Link to article on publisher's site</a></p>0027-8424 (Linking)10.1073/pnas.140310111124979786https://hdl.handle.net/20.500.14038/26464Outer arm dynein (OAD) in cilia and flagella is bound to the outer doublet microtubules every 24 nm. Periodic binding of OADs at specific sites is important for efficient cilia/flagella beating; however, the molecular mechanism that specifies OAD arrangement remains elusive. Studies using the green alga Chlamydomonas reinhardtii have shown that the OAD-docking complex (ODA-DC), a heterotrimeric complex present at the OAD base, functions as the OAD docking site on the doublet. We find that the ODA-DC has an ellipsoidal shape approximately 24 nm in length. In mutant axonemes that lack OAD but retain the ODA-DC, ODA-DC molecules are aligned in an end-to-end manner along the outer doublets. When flagella of a mutant lacking ODA-DCs are supplied with ODA-DCs upon gamete fusion, ODA-DC molecules first bind to the mutant axonemes in the proximal region, and the occupied region gradually extends toward the tip, followed by binding of OADs. This and other results indicate that a cooperative association of the ODA-DC underlies its function as the OAD-docking site and is the determinant of the 24-nm periodicity.en-US<p>Publisher PDF posted as allowed by the publisher's author rights policy at http://www.pnas.org/site/aboutpnas/authorfaq.xhtml.</p>AxonemeBlotting, WesternChromatography, GelDyneinsElectrophoresis, Polyacrylamide GelElectroporationFluorescent Antibody TechniqueMacromolecular SubstancesMicroscopy, ElectronMicroscopy, FluorescenceMicrotubules*Models, BiologicalProtein BindingRosaniline DyesUltracentrifugationAmino Acids, Peptides, and ProteinsBiochemistryCell and Developmental BiologyCell BiologyCellsEnzymes and CoenzymesGenetic PhenomenaInvestigative TechniquesJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1148&amp;context=cellbiology_pp&amp;unstamped=1https://escholarship.umassmed.edu/cellbiology_pp/1496929799cellbiology_pp/149