Lopes, Magno AlvesOliveira Franco, FelipeHenriques, FelipePeres, Sidney BarnabeBatista, Miguel Luiz Jr.2022-08-232022-08-232018-07-302018-09-13<p>Heliyon. 2018 Jul 30;4(7):e00708. doi: 10.1016/j.heliyon.2018.e00708. eCollection 2018 Jul. <a href="https://doi.org/10.1016/j.heliyon.2018.e00708">Link to article on publisher's site</a></p>2405-8440 (Linking)10.1016/j.heliyon.2018.e0070830094378https://hdl.handle.net/20.500.14038/40739Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established pre-adipocyte cell line 3T3-L1 adipogenic capacity. c/c-LLC in the presence of 3T3-L1 caused a reduction in lipids accumulation, suggesting that secretory tumor cells products may affect adipogenesis. Interestingly, a very early (day 2) down-regulation of proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha (C/EBPalpha), followed by late genes (day 4 and 8), adiponectin, perilipin, and fatty acid-binding protein 4 (FABP4). Caspase-3 expression was increased on the last day of cell differentiation; it occurred in the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Overall, our results suggest that LLC secretory products impair adipocyte differentiation in a co-culture system and increased apoptosis. In summary, our study has shown the inhibition of the adipogenic process in the 3T3-L1 co-culture system with LLC cells.en-USCopyright 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/Cancer researchCell biologyMolecular biologyAmino Acids, Peptides, and ProteinsCancer BiologyCell BiologyCellsCellular and Molecular PhysiologyDevelopmental BiologyGenetic PhenomenaMolecular BiologyLLC tumor cells-derivated factors reduces adipogenesis in co-culture systemJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4556&context=oapubs&unstamped=1https://escholarship.umassmed.edu/oapubs/354412835197oapubs/3544