Leitch, Jeffry M.Carruthers, Anthony2022-08-232022-08-232007-02-132009-02-18Am J Physiol Cell Physiol. 2007 Feb;292(2):C974-86. Epub 2006 Aug 23. <a href="http://dx.doi.org/10.1152/ajpcell.00335.2006">Link to article on publisher's site</a>10.1152/ajpcell.00335.200616928769https://hdl.handle.net/20.500.14038/26122Human erythrocyte glucose sugar transport was examined in resealed red cell ghosts under equilibrium exchange conditions ([sugar](intracellular) = [sugar](extracellular), where brackets indicate concentration). Exchange 3-O-methylglucose (3MG) import and export are monophasic in the absence of cytoplasmic ATP but are biphasic when ATP is present. Biphasic exchange is observed as the rapid filling of a large compartment (66% cell volume) followed by the slow filling of the remaining cytoplasmic space. Biphasic exchange at 20 mM 3MG eliminates the possibility that the rapid exchange phase represents ATP-dependent 3MG binding to the glucose transport protein (GLUT1; cellular [GLUT1] ofen-USJournal Articlehttps://escholarship.umassmed.edu/bmp_pp/61724049bmp_pp/61