Ace, Christopher I.Okulicz, William C.2022-08-232022-08-231998-10-132008-10-31<p>J Clin Endocrinol Metab. 1998 Oct;83(10):3569-73.</p>0021-972X (Print)10.1210/jcem.83.10.51509768666https://hdl.handle.net/20.500.14038/42656We have previously prepared and characterized a subtracted library enriched for endometrial progesterone (P)-dependent genes in the rhesus monkey. One of the fragment clones (H3) that we selected for sequencing from this library was found to be homologous to human DMBT1, a recently isolated member of the scavenger receptor cysteine-rich superfamily and a new putative tumor suppressor. In this report, we provide evidence that H3 is the rhesus monkey homolog of DMBT1. Additional sequence data of H3 (1071 bp) showed a striking homology with DMBT1 (92% identical). Semiquantitative kinetic PCR of estrogen-dominant vs. P-dominant endometrial complementary DNA populations showed that the H3 gene was up-regulated 5-fold by normal secretory P levels. In situ hybridization with unique probes to H3 confirmed the up-regulation by P in the endometrium and a restricted expression in the stromal compartment. Another recent report suggested the presence of an endometrial tumor suppressor in the same chromosomal region as DMBT1 (10q23-26); deletions in this region were associated with endometrial cancers. Together, these studies potentially provide a molecular link to the protective effect of the action of P on unopposed estrogen exposure in reproductive tract cancers in women.en-US*AgglutininsAmino Acid SequenceAnimalsEndometriumFemaleGene ExpressionGenes, Tumor SuppressorHumansIn Situ HybridizationMacaca mulattaMolecular Sequence DataPolymerase Chain ReactionProgesteroneReceptors, Cell SurfaceSequence Homology, Amino AcidLife SciencesMedicine and Health SciencesJournal Articlehttps://escholarship.umassmed.edu/oapubs/984659167oapubs/984