Yin, JunGibbs, MaryLong, CaixiaRosenthal, JustinKim, Hyong S.Kim, AnnaSheng, ChengyuDing, PengJaved, UzmaYuan, Quan2022-08-232022-08-232018-10-302018-12-12<p>Cell Rep. 2018 Oct 30;25(5):1181-1192.e4. doi: 10.1016/j.celrep.2018.10.016. <a href="https://doi.org/10.1016/j.celrep.2018.10.016">Link to article on publisher's site</a></p>2211-1247 (Electronic)10.1016/j.celrep.2018.10.01630380410https://hdl.handle.net/20.500.14038/40847Activity-dependent modifications strongly influence neural development. However, molecular programs underlying their context and circuit-specific effects are not well understood. To study global transcriptional changes associated with chronic elevation of synaptic activity, we performed cell-type-specific transcriptome profiling of Drosophila ventral lateral neurons (LNvs) in the developing visual circuit and identified activity-modified transcripts that are enriched in neuron morphogenesis, circadian regulation, and lipid metabolism and trafficking. Using bioinformatics and genetic analyses, we validated activity-induced isoform-specific upregulation of Drosophila lipophorin receptors LpR1 and LpR2, the homologs of mammalian low-density lipoprotein receptor (LDLR) family proteins. Furthermore, our morphological and physiological studies uncovered critical functions of neuronal lipophorin receptors (LpRs) in maintaining the structural and functional integrities in neurons challenged by chronic elevations of activity. Together, our findings identify LpRs as molecular targets for activity-dependent transcriptional regulation and reveal the functional significance of cell-type-specific regulation of neuronal lipid uptake in experience-dependent plasticity and adaptive responses.en-USThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).http://creativecommons.org/licenses/by-nc-nd/4.0/RNA-seq analysisactivity-dependent transcriptional regulationdendrite morphogenesislipid homeostasislipid uptakelipoprotein receptorneural developmentneuronal adaptationstructural plasticitytranscriptome profilingAmino Acids, Peptides, and ProteinsLipidsNeuroscience and NeurobiologyNucleic Acids, Nucleotides, and NucleosidesJournal Articlehttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=4665&amp;context=oapubs&amp;unstamped=1https://escholarship.umassmed.edu/oapubs/365313473108oapubs/3653