Hassan, Mohammad Q.Gordon, Jonathan A. R.Lian, Jane B.Van Wijnen, Andre J.Stein, Janet L.Stein, Gary S.2022-08-232022-08-232010-06-222010-08-03J Cell Biochem. 2010 Jul 1;110(4):817-22. <a href="http://dx.doi.org/10.1002/jcb.22562">Link to article on publisher's site</a>0730-2312 (Linking)10.1002/jcb.2256220564179https://hdl.handle.net/20.500.14038/26585We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.en-USImmunoprecipitationRibonucleoproteinsMicroRNAsCell BiologyJournal Articlehttps://escholarship.umassmed.edu/cellbiology_pp/921421243cellbiology_pp/92